The historical temperature and phenological data were analysed for the region of Sremski Karlovci, one of the oldest grapevine growing areas in Europe, with the aim of detecting trends of changes in the data, evaluating the sensitivity of grapevine phenology to temperature and revealing diversity among cultivars in their response to observed changes in temperature. The onset dates of four major phenological stages (budburst, flowering, veraison and harvest), along with the corresponding growth intervals between them, were examined for 20 wine grape cultivars. A number of climatically important parameters for viticulture were calculated for the calendar year, growing season and different grapevine growth periods. Significant increases were detected in average and heat-related extreme temperature indices. The greatest rate of change in temperature variables across the growing season was observed during the period from the beginning of flowering to the beginning of veraison and the smallest during the ripening period. Linear trends indicated that all phenological stages, except budburst, have advanced significantly. Averaged across all cultivars, detected trends were -0·4, -0·7 and -0·6 days/year for the beginning of flowering, the beginning of veraison and harvest date, respectively. Observed warming and change in the timing of phenological events did not significantly affect the duration of the growth intervals, which can be explained by significant inter-correlation between the phenological stages' onset. Ripening was occurring under warmer conditions due to earlier flowering and veraison, rather than because of considerably higher temperatures preceding harvest or shortening of the ripening period. Most of the variation in phenology timing (74-90%) can be explained by a linear relationship between the onset date of phenological stage and temperature, with mean and maximum temperatures being more important than minimum temperatures. According to the current results, a 1°C increase in the most influential temperature variable during the most relevant periods for the onset of phenological stages led to an advancement in the beginning of budburst, the beginning of flowering, the beginning of veraison and harvest by 3·6, 3·1, 5·2 and 7·4 days, respectively, on average for all cultivars. Among the cultivars studied, Pinot Noir displayed the greatest phenology advancement in response to increased temperature.

No abstract

Over the past two centuries, introgression through repeated backcrossing has introduced disease resistance from wild grape species into the domesticated lineage Vitis vinifera subsp. sativa. Introgression lines are being cultivated over increasing vineyard surface areas, as their wines now rival in quality those obtained from preexisting varieties. There is, however, a lot of debate about whether and how wine laws defining commercial product categories, which are based on the classification of V. vinifera and interspecific hybrid grapes, should be revised to accommodate novel varieties that do not fit either category. Here, we developed a method of multilocus genotype analysis using short–read resequencing to identify haplotypic blocks of wild ancestry in introgression lines and quantify the physical length of chromosome segments free–of–introgression or with monoallelic and biallelic introgression. We used this genomic data to characterize species, hybrids and introgression lines and show that newly released resistant varieties contain 76.5–94.8% of V. vinifera DNA. We found that varietal wine ratings are not always commensurate with the percentage of V. vinifera ancestry and linkage drag of wild alleles around known resistance genes persists over at least 7.1–11.5 Mb, slowing down the recovery of the recurrent parental genome. This method also allowed us to identify the donor species of known resistance haplotypes, define the ancestry of wild genetic background in introgression lines with complex pedigrees, validate the ancestry of the historic varieties Concord and Norton, and unravel sample curation errors in public databases.

Aim: Leaf removal around clusters and cluster thinning are techniques usually applied in cool-climate vineyards in order to achieve optimal grape maturity. However, the impact of the timing of these two operations differs across varieties. Thus, the aim of the present work was to investigate the effects of cluster thinning and leaf removal timing (performed at three specific time points) on grape quality and monomeric anthocyanins in the wines of Cabernet-Sauvignon and Probus (Kadarka × Cabernet-Sauvignon, Vitis vinifera L.)Methods and results: The experiment was conducted in Sremski Karlovci (Northern Serbia) in 2014, 2015, and 2016. Leaf removal was applied on six basal nodes of each shoot at three time points, 7 days after flowering, 30 days after flowering, and at veraison, i.e., at the onset of berry ripening. After cluster thinning, which was performed 7 days after flowering, one cluster per shoot was retained. On the treated vines, leaf removal treatment and cluster thinning were applied only once. Leaf removal was more effective than cluster thinning in respect to grape quality. Leaf removal, applied 7 and 30 days after flowering, decreased titratable acidity in Cabernet-Sauvignon, while in Probus an interaction of leaf removal and year was observed. Moreover, early leaf removal decreased the incidence of Botrytis sp. in Probus. The varieties reacted differently to cluster thinning in respect to grape quality: cluster thinning increased total soluble solids in Probus and lowered titratable acidity in Cabernet-Sauvignon. In 2015, both cluster thinning and leaf removal yielded changes in the anthocyanin ratios in the wines. Cluster thinning increased total and acylated anthocyanins in the wine of Cabernet-Sauvignon compared to wine derived from unthinned vines. The peonidin content was 40 % higher in the Cabernet-Sauvignon wine if the vines were subjected to leaf removal treatments.Conclusions: Cluster thinning and leaf removal affected both Cabernet-Sauvignon and Probus (Vitis vinifera L.) grape quality and wine composition. Early leaf removal was the most effective treatment in both varieties. Therefore, combined application of cluster thinning and early leaf removal is highly recommended in the production of high-quality red wines in Serbia.Significance and impact of the study: Timing of leaf removal application was usually investigated around flowering and veraison. Our results suggested that leaf removal between these two phenological stages also improves grape quality and changes the ratio of the monomeric anthocyanins in the wine.

The use of nutritional strategies to improve the quality of meat is a relatively new approach that has emerged at the interface of animal science and food science. The effects of dietary medicinal plants (Allium sativum L., Piper nigrum L., and Capsicum annuum L.) addition to chicken nutrition on quality characteristics of breast and thigh with drumstick meat, as well as caloric value of chicken meat were investigated. Quality measurements included meat sensory (colour, smell, taste, softness, chewiness, juiciness and overall impression), physical (pH, colour-CIE L*a*b* and drip-loss) and chemical (moisture, protein, fat and ash content) characteristics. Herbs showed significant (P < 0.05) influence in altering most examined quality parameters of chicken meat, especially when adding red hot pepper. Caloric value of chicken meat was improved which makes garlic, black pepper and hot red pepper valuable natural feed additives in improvement of meat quality as well as a natural growth promoter. In conclusion, herbs had positive influence on chicken meat quality, however the knowledge of their mode of action is still limited and thus requires further investigation.