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A UV spectrophotometric and RP-HPLC method for the Naringenin quantification in the developed solid dispersions is described. Both the methods were validated according to ICH Q2 R (1) guidelines for linearity, accuracy, precision, limit of detection, limit of quantification, specificity and robustness. HPLC was run in isocratic mode on a reversed-phase C18 column (250 × 4.6 mm internal diameter and particle size of 5 μm) with methanol:water (70:30, v/v) as the mobile phase maintaining a flow rate of 1.0 ml/min. Naringenin showed an absorbance maximum (λ max) at 288 nm which was used for the UV spectrophotometric determinations. The calibration curve of Naringenin showed linearity in the required concentration range (R 2 > 0.999) by both the UV and HPLC methods. Both the methods were found to be precise and accurate with recovery range of 98-101% and relative standard deviation < 2%. Most importantly, the accuracy and precision achieved by the HPLC method, correlated closely with the UV method. Current study also involves detection and quantification of Naringenin released from its formulations by both the developed methods. This paper demonstrates the high correlation (R 2 ≥ 0.98) between the UV and HPLC methods when determining the release of Naringenin from various formulations. With this established correlation, we hereby suggest that, for routine analysis, UV spectroscopy can be an economic, simple, reliable and less time-consuming alternative for expensive and time-consuming chromatographic analysis.

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