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Spray drying is appropriate for the preservation of halophilic microorganisms due to the nature of these microorganisms, as they survive in adverse environmental conditions by being encapsulated in salt crystals. Artificial neural networks were in this study used to optimize practically significant spray-drying regimes of the C-carotenoids producer Halobacterium salinarum. Immediately after drying, the samples contained up to 54% halobacterial biomass and less than 5% moisture, and the level of preservation of carotenoids was 95-97%. The storage of biomass at 4 °C resulted in the gradual degradation of the carotenoids, which reached 58-64% in the best samples after 1 year. A comprehensive study of changes in halobacteria biomass after spray drying and the nature of the damage provided new data on the survival and preservation of cells and biologically active substances in the various spray-drying regimes and at different storage times.

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The dynamics of Leuconostoc mesenteroides bacteria growth and synthesis of exoplysaccharides when cultured on a medium containing whey and sucrose has been investigated. It was shown that exopolysaccharides were produced during the culture growth and the addition of sucrose increased in the productivity of those compounds without affecting their composition. Using the dynamic and static methods of light scattering, it was shown that sucrose as a medium component modified physicochemical properties of exopolysaccharides changing their structural and thermodynamic characteristics in aqueous medium, which affected their functional activity and determined the way of their most efficient use. kefir fungi, lactic acid bacteria, physicochemical properties of exopolysaccharides.