Hyperthermia is a performance and welfare issue for exercising horses. The thermoregulatory stressors associated with exercise have typically been estimated by responses in the laboratory. However, monitoring surface skin temperature (Tsk) coincident with core temperature (Tc) has not previously been investigated in horses exercising in the field. We investigated the suitability of monitoring surface Tsk as a metric of the thermoregulatory response, and simultaneously investigated its relationship with Tc using gastrointestinal (GI) temperature. We evaluated Tsk in 13 endurance horses competing during four endurance rides over 40 km (n = 1) or a total of 80 km (n = 12) distance. Following each 40-km loop, the horses were rested for 60 min. Tsk and Tc were continuously recorded every 15 s by an infrared thermistor sensor located in a modified belt and by telemetric GI pill, respectively, and expressed as mean ± SD. The net area under the curve (AUC) was calculated to estimate the thermoregulatory response to the thermal load of Tsk over time (°C × minutes) using the trapezoidal method. The relationship between Tsk and Tc was assessed using scatterplots, paired t-test or generalized linear model ANOVA (delta Tsk) (n = 8). Ambient temperature ranged from 6.7°C to 18.4°C. No relationship was found between Tsk and Tc profiles during exercise and recovery periods, and no significant difference between delta Tsk results was detected when comparing exercise and rest. However, time to maximum Tsk (67 min) was significantly reduced compared to Tc (139 min) (p = 0.0004) with a significantly lesser maximum Tsk (30.3°C) than Tc (39°C) (p = 0.0002) during exercise. Net AUC Tsk was 1,164 ± 1,448 and −305 ± 388°C × minutes during periods of exercise and recovery, respectively. We conclude that Tsk monitoring does not provide a reliable proxy for the thermoregulatory response and horse welfare, most probably because many factors can modulate Tsk without directly affecting Tc. Those factors, such as weather conditions, applicable to all field studies can influence the results of Tsk in endurance horses. The study also reveals important inter-individual differences in Tsk and Tc time profiles, emphasizing the importance of an individualized model of temperature monitoring.

OBJECTIVE To evaluate use of a telemetric gastrointestinal (GI) pill to continuously monitor GI temperature in horses at rest and during exercise and to compare time profiles of GI temperature and rectal temperature. ANIMALS 8 Standardbred horses. PROCEDURES Accuracy and precision of the GI pill and a rectal probe were determined in vitro by comparing temperature measurements with values obtained by a certified resistance temperature detector (RTD) in water baths at various temperatures (37°, 39°, and 41°C). Subsequently, both GI and rectal temperature were recorded in vivo in 8 horses over 3 consecutive days. The GI temperature was recorded continuously, and rectal temperature was recorded for 3.5 hours daily. Comparisons were made between GI temperature and rectal temperature for horses at rest, during exercise, and after exercise. RESULTS Water bath evaluation revealed good agreement between the rectal probe and RTD. However, the GI pill systematically underestimated temperature by 0.14°C. In vivo, GI temperature data were captured with minimal difficulties. Most data loss occurred during the first 16 hours, after which the mean ± SD data loss was 8.6 ± 3.7%. The GI temperature was consistently and significantly higher than rectal temperature with an overall mean temperature difference across time of 0.27°C (range, 0.22° to 0.32°C). Mean measurement cessation point for the GI pill was 5.1 ± 1.0 days after administration. CONCLUSIONS AND CLINICAL RELEVANCE This study revealed that the telemetric GI pill was a reliable and practical method for real-time monitoring of GI temperature in horses.

Establishing proper policies regarding the recognition and prevention of equine heat stress becomes increasingly important, especially in the face of global warming. To assist this, a detailed view of the variability of equine thermoregulation during field exercise and recovery is essential. 13 endurance horses and 12 trotter horses were equipped with continuous monitoring devices [gastrointestinal (GI) pill, heartrate (HR) monitor, and global positioning system] and monitored under cool weather conditions during four endurance rides over a total of 80 km (40 km loops) and intense trotter track-based exercise over 1,540 m. Recordings included GI temperature (Tc), speed, HR and pre- and post-exercise blood values. A temperature time profile curve of Tc was constructed, and a net area under the curve was calculated using the trapezoidal method. Metabolic heat production and oxygen cost of transport were also calculated in endurance horses. Maximum Tc was compared using an independent samples t-test. Endurance horses (mean speed 14.1 ± 1.7 km h–1) reached mean maximum Tc (39.0 ± 0.4°C; 2 × 40 km in 8 horses) during exercise at 75% of completion of Tc exercise and Tc returned to baseline within 60 min into recovery. However, the mean Tc was still 38.8 ± 0.4°C at a HR of 60 bpm which currently governs “fit to continue” competition decisions. Trotters (40.0 ± 2.9 km h–1) reached a comparable mean max Tc (38.8 ± 0.5°C; 12 horses) always during recovery. In 30% of trotters, Tc was still >39°C at the end of recovery (40 ± 32 min). The study shows that horses are individuals and thermoregulation monitoring should reflect this, no matter what type of exercise is performed. Caution is advised when using HR cut-off values to monitor thermal welfare in horses since we have demonstrated how Tc can peak quite some time after finishing exercise. These findings have implications for training and management of performance horses to safeguard equine welfare and to maximize performance.