Objectives: The aim of this study was to evaluate the hepatoprotective activity of resveratrol in patients with morphofunctional liver disorders due to mechanical jaundice. Methods: The controlled mechanical jaundice was simulated on the rats under anesthesia by drainage of the choledochus and its plugging. In the control group decompression of the choledochus was performed on the 3rd day and intravenous infusion of 0,9% sodium chloride solution was administrated for 12 days. In the experimental group after billiar decompression, resveratrol was administered intravenously at a dose of 20 mg/kg. The following were studied: level of malondialdehyde, catalase, bilirubin, alanine aminotransferase (ALAT), aspartate aminotransferase; liver histology was performed. Results: Catalase was 3.9 times reliably increased, and the activity of malonic dialdehyde and ALAT was 2.0 and 3.6 times reliably decreased in experimental group compared to the control group. Microscopic shows edema and destruction of the liver were reduced, the architectonic of the liver lobules was recovered. Conclusion: Application of resveratrol in mechanical jaundice decreased the processes of free-radical oxidation and level of the liver cells cytolysis markers which morphologically was shown by edema reduction, restoration of the liver lobules structure.

In this paper, we consider a relation between adolescent obesity and dynamic changes in the complement system components and immune complexes of various fractions affected by antihypoxants, because obesity is one of the most common non-communicable diseases worldwide and rises an interest for many researchers. New studies emerge revealing an imbalanced immune system that aggravates upon obesity and affects multiple immune processes, as well as increasing interest in studying obstructive sleep apnea syndrome resulting in tissue hypoxia, thereby increasing a risk of cardiovascular diseases. Our study allowed to detect metabolic syndrome in 89% of obese patients (groups 1 and 2) and 25% of adolescents with normal body weight (control group 2). We analyzed the complement components and circulating immune complexes of various fractions and revealed positive dynamics of the examined parameters due to combination therapy with the anti-oxidant Mexidol and hyperbaric oxygenation, which may indicate that oxygen-dependent neuroimmune mechanisms might be involved in the pathogenesis of metabolic syndrome in obesity. Such systemic activation of regulatory mechanisms for maintaining homeostasis is necessary for formation of biologically active thymusderived serum factor ensuring lymphocyte differentiation, i.e. pathogenetic therapy corrects dysregulatory impact of traditional therapy for obesity and optimizes immune responses. During the study we observed that the combination treatment using Mexidol and HBO resulted in better recovery of clinical functions and immune responses, and that such therapy exerted a positive effect on dynamics of patient mental activity. We believe that such therapeutic effects were due to antioxidant and antihypoxic activity of the applied pharmacological drugs. We also found that obese children are featured with metabolic syndrome and predisposed to developing relevant complications. Hence, for early diagnostics of metabolic syndrome it is necessary to study children with normal body weight that will allow to diagnose metabolic changes at earlier stage, because changes in blood biochemical parameters such as triglycerides, very low-density lipoproteins, glycemia levels etc. occur long before initial changes in person appearance might emerge.

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Ïå÷åíü / LiverЦель. Оценка эффективности отрицательных аэроионов в устранении печеночной дисфункции при механической желтухе. Материал и методы. Проведены 2 серии экспериментов на 30 крысах. В 1-й (контрольной) серии моделировали механическую желтуху с последующей декомпрессией общего желчного протока и введением 0,9% раствора хлорида натрия в объеме 40 мл/кг. Во 2-й (опытной) серии воздействовали отрицательными аэроионами (3 млн частиц в 1 см 3 с экспозицией 60 мин 1 раз в сутки на протяжении 12 дней). Изучали уровень билирубина, малонового диальдегида, активность АлАТ и АсАТ, щелочной фосфатазы, каталазы. Результаты. Применение отрицательных аэроионов при механической желтухе способствовало более существенной коррекции показателей функциональной активности печени. Концентрация общего и прямого билирубина уменьшалась до 84,4 ± 7,6 и 42,1 ± 5,4 мкмоль/л, что на 25 и 56% меньше, чем в контрольной серии. Меньше показателей контрольной серии была активность АсАТ и АлАТ, щелочной фосфатазы, концентрация малонового диальдегида (на 17, 22, 18, 47%) и в 3,6 раза больше -активность каталазы. Заключение. Применение отрицательных аэроионов при механической желтухе стимулирует выработку собственных антиоксидантных ферментов, защищающих гепатоциты от свободнорадикального повреждения.