No abstract

No abstract

The current intensive industrialization has changed the food preferences of consumers. As a result, there is a growing demand for high-grade high-nutritional meat and dairy products, which, in its turn, triggered an increase in the demand for grain crops and led to higher animal feed prices. All these affected the price and quality of the finished product, since farms are trying to stay profitable. As a consequence, the high cost of animal proteins make producers look for other sources of protein with similar qualities. Common oat (Avena sativa L.) remains the most cultivated species. Oats are a source of high-quality protein with an optimal amino acid balance. The paper features a oat protein technology (Avena sativa). The research defined the parameters of the protein extraction process. For acid and alkaline methods, the following optimum parameters were revealed: temperature – 40 ± 2°C, hydraulic module – 1:10, time – 90 minutes, active acidity of the acid extraction – 2.0 units, active acidity of alkaline extraction – 9.0 units. The authors managed to obtain protein substances with the molecular weight > 50 kDa. The optimal parameters of ultrafiltration of the protein extract were as follows: pore diameter = 100 kDa at pH 8.0 and 0.5 MPa. The ultrafiltration conducted under these conditions showed that the content of high molecular fractions (globulins and albumins) increased from 39.12% to 55.15% for the extract obtained by alkaline method, whereas the content of low molecular weight fractions (prolamins and glutelins) decreased from 60.88% to 44.85%. Ultrafiltration of protein extracts obtained by alkaline and acidic methods made it possible to concentrate protein fractions with a molecular weight ≥ 50 kDa. When a 10% aqueous solution of succinic acid was used as a precipitator, the protein precipitation degree equaled 89.3%. The paper introduces a new oat protein purification method. The optimal multiplicity of purification by RP-HPLC was 4 purification cycles. For the alkaline extract, the total content of high molecular weight fractions (50.0–120.0 kDa) was 72.7% and the total content of low molecular weight fractions (15.0–49.0 kDa) was 27.3%. For the acid extract, the total content of high molecular weight fractions was 72.9%, while the content of low molecular weight fractions was 27.1%. Oat proteins obtained by alkaline and acid extraction demonstrated a high foaming ability (148–177%) at pH = 6.0–9.0, as well as a good fat and water retention capacity. The oat proteins were found to have a high content of protein and essential amino acids similar to animal proteins. A comparative analysis showed that oat protein can act as an alternative substitute for animal proteins.

The industry of sport nutrition is actively developing worldwide and includes a wide range of functional products. Healthy lifestyle is being actively promoted, hence the development of new types of products for athletes. Protein mixtures and amino acid complexes occupy a leading position in this market sector. Nutrition plays an important role in physical training, since athletic activity requires a lot of energy and causes neuro-psychological stress. A standard diet cannot meet the needs of athletes, so this group of people uses fortified foods and biologically active food supplements. Today, sport nutrition is widely represented on the shelves of mass markets. However, most of the products are imported. The qualitative composition of the protein is important in determining its suitability for human diet. Products of animal origin have been a traditional source of protein in the diet and, despite its balanced amino acid composition, still have several disadvantages. Plant sources of protein have recently attracted the interest of scientists and nutritionists. Such products possess sufficient biological potential and are more affordable for production and processing. Oats is actively used in the food industry due to its beneficial properties. The present paper features the biological potential of the curd product fortified with an oat protein concentrate and its role in sport nutrition. The paper introduces a new technology for the production of a curd product fortified with an oat protein concentrate. The finished product contains 23.5% of protein and all essential amino acids, which meets the requirements of sport nutrition. A comparative analysis of the content of amino acids in popular sport nutrition products and the developed curd product proved the competitiveness of the latter. Its biological potential appeared to be not inferior to the characteristics of popular commercial products. The curd product fortified with an oat protein concentrate demonstrated a balanced amino acid, vitamin, macro, and microelement composition, which makes it possible to recommend it as a functional food product for people who are actively involved in sports.

Recently, the market of sport nutrition has been expanding due to high popularity of sports in human everyday life as well as popularity of healthy lifestyle principles. This article offers to consider and validates main stages to produce functional sport products based on oat grains. The production technology includes the following stages: Acceptance and quality evaluation of the raw stock; interim storage and preparation of raw stock for production stage; oat grain grinding; protein extraction from the raw stock; ultrafiltration; protein sedimentation; addition of vitamin and mineral mixture and cocoa powder; mixing; packing, labeling and storage of the end product. It is found that the greatest volume protein is extracted when the aqueous solution of 1 M sodium hydroxide is used (protein volume totaled to 93.65 ± 5.62%) at the similar technical parameters of acidic and alkali extraction. Ultrafiltration used to concentrate proteins with UPM-100 membrane contributes to the maximum degree of protein concentration (3.50 ± 0.21%). The protein is sedimented by the 10% aqueous solution of amber acid at the rate of active acidity of 4.4 units that allows an increase in the extent of protein sedimentation to 86.9 ± 5.21%. The end product obtained as per the scheme submitted has been studied for its organoleptic, physical and chemical and microbiological properties, including its blend composition. It’s been identified that the food product has pure taste and flavor appropriate of this product, with no foreign flavor and aroma. High content of protein, Omega-3 and Omega-6 fatty acids, as well as all essential amino acids are reported. The daily portion of the developed functional product contains 46.0% of vitamins and mineral substances against the rate of physiological need in them.