Plastic pollution has been documented across the Laurentian Great Lakes basin. Here, we demonstrate the application of a published ecological risk assessment and management framework for microplastics in aquatic environments by comparing proposed thresholds for risk to monitoring data from the Great Lakes region. Our results suggest that there may be measurable risks from microplastics to aquatic communities in parts of the Great Lakes where current concentrations are relatively high. For example, 89% of surface water samples collected across the region exceed the proposed risk thresholds for food dilution toxicity. However, all sediment samples remain below the proposed risk thresholds. Accordingly, a necessary and appropriate next step may include convening a workgroup of local experts to develop ecological risk assessment and management frameworks for the region comprising thresholds of concern for microplastics in surface water and sediment. Ultimately, microplastic pollution should be addressed in the Great Lakes Water Quality Agreement to ensure coordinated and sustained efforts are taken by the governments of Canada and the United States to reduce their release and impact.