RESUMO -Para determinação da dose inseminante ideal na fertilização artificial de ovócitos de jundiá cinza (Rhamdia quelen), foram selecionadas duas fêmeas com papila urogenital avermelhada, abdômen arredondado e liberação de ovócitos de cor e tamanho uniforme e quatro machos que apresentavam liberação de sêmen sob pressão da papila genital. As fêmeas e os machos receberam duas doses de extrato de hipófise de carpa (doses inicial e final de 0,5 e 0,25 mg.kg -1 e de 5,0 e 2,5 mg.kg -1 , para fêmeas e machos, respectivamente). As duas fêmeas produziram 169 g de ovos contendo 1.141 ovócitos.g de ovócitos -1 e os machos, um total de 23,6 mL de sêmen. Do sêmen dos quatro machos foi realizado um pool e analisadas as seguintes características: concentração espermática (1,97x10 10 espermatozóides.mL -1 ), índice de sobrevivência espermática (96%) e morfologia espermática (66,9% de espermatozóides normais). No ensaio de fertilização, utilizou-se delineamento experimental inteiramente casualisado, composto de cinco tratamentos (6x10 3 , 6x10 4 , 6x10 5 , 6x10 6 , 2x10 7 espermatozóides.ovócito -1 ) e quatro repetições. Após 8 horas de incubação, foram analisadas as taxas de fertilização para cada tratamento. A melhor relação espermatozóide.ovócito -1 foi de 89.497 espermatozóide.ovócito -1 , o que provocou taxa de fertilização máxima teórica de 86,68%. Palavras-chave: dose inseminante, reprodução, Rhamdia quelen Insemination dose for artificial fertilization of grey jundia oocytes, Rhamdia quelen (Quoy & Gaimardm, 1824)ABSTRACT -Two females with colored urogenital organ, rounded off abdomen, color egg released, and uniform size and four males with semen released under abdomen pressure were selected to determine the best insemination dose for artificial fertilization of grey jundia, Rhamdia quelen (Quoy & Gaimardm, 1824), oocytes. Females and males received two doses of carp pituitary extract: the first one of 0.5 and 0.25 mg.kg -1 and the second of 5.0 and 2.5 mg.kg -1 for females and males, respectively. Both females produced 169 g of eggs with 1,141 oocytes.oocytes g -1 . The males produced a total of 23.6 mL of semen. From all males semen pool sperm concentration (1.97 x 10 10 spermatozoa.mL -1 ), survival sperm index (96%) and sperm morphology were analyzed (66.9% of normal spermatozoa). The fertilization assay was conducted as a complete randomized experimental design with five treatments ((6x10 3 , 6x10 4 , 6x10 5 , 6x10 6 , 2x10 7 spermatozoa.oocyte -1 ) and four replications. After eight hours of incubation, the fertilization rates for each treatment were analyzed. The best spermatozoa.oocyte -1 rate was of 89.497 spermatozoa.oocyte -1 that produced a maximum fertilization rate of 86.68%.
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