BACKGROUND: Higher consumption of phenolic-antioxidant rich berries have been associated with lower risks of chronic diseases, such as type 2 diabetes. In order to target for diet-based therapy it is important to screen and evaluate the right cultivars of berries with optimum phenolic antioxidants and associated anti-hyperglycemic functions. OBJECTIVE: Therefore, the primary objective of this study was to screen different rabbit-eye blueberry cultivars extracted in water and ethanol for their phenolic antioxidant-linked anti-diabetic properties using in vitro assays. Further impact of cultivars, growing seasons, and locations on targeted human health relevant bioactive profiles were also evaluated. METHODS: Water and ethanol extracts of five rabbit-eye blueberry cultivars from two different locations and from two different growing seasons were evaluated for phenolic acids profile, total soluble phenolic content, total antioxidant activity, ␣-amylase, ␣-glucosidase, and angiotensin I-converting enzyme (ACE) inhibitory activities using model in vitro assays. RESULTS: Significant variations in phenolic antioxidant-linked anti-hyperglycemic functions were observed due to differences in genotypes, growing seasons, and locations. High phenolic antioxidant-linked anti-hyperglycemic functions were observed in Tiffblue, Brightwell, and Primier rabbit-eye blueberry cultivars and indicated anti-diabetic potential. CONCLUSION: Genotype × environment interactions are most critical factors which determined in vitro anti-diabetic relevant functionalities of blueberry bioactives.
Kiwifruit is a new emerging crop for the southeastern United States that requires cross-pollination to set fruit. However, the pollination requirements for varieties grown in the southeastern United States are unknown. Through insect surveys and a bagging experiment, we assessed the pollination requirements of two female kiwifruit cultivars (Actinidia chinensis var. chinensis ‘AU Golden Sunshine’ and A. chinensis var. chinensis ‘AU Gulf Coast Gold’). For each, fruit quantity (fruit set) and fruit quality (weight, size, seed count, firmness, soluble solid content, and dry matter) were compared among three pollination treatments (wind, insect, and artificial pollination). Low abundances of insects were observed visiting female flowers of both kiwifruit cultivars, and therefore likely minimally influenced kiwifruit pollination. Artificial pollination resulted in the greatest percentages of fruit set and marketable fruits, followed by insect and wind pollination. Artificial pollination resulted in fruits that were greater in weight, size, and contained more seeds, than insect- and wind-pollinated fruits. Firmness and soluble solid content did not vary greatly between pollination treatments, yet were greater in ‘AU Golden Sunshine’. Dry matter content did not vary greatly between pollination treatments or between each cultivar. To maximize yields and optimize fruit quality, these results suggest that kiwifruit producers should place more effort into artificial pollination compared to wind and insect pollination. Future research should explore the use of managed bees (e.g., honey bees and bumble bees) within kiwifruit orchards to determine ways to utilize them as a secondary source for pollination needs.
Stink bugs (Hemiptera: Pentatomidae) are commonly monitored using pyramid traps baited with a pheromone. Initially, the pyramid traps were painted yellow and predominantly used to monitor native stink bug species. However, research studies involving the exotic Halyomorpha halys Stål (Hemiptera: Pentatomidae) now use pyramid traps that are black, not yellow. As H. halys moves across the southeastern United States, the use of a single trap, yellow or black, for monitoring and conducting research studies would be beneficial. Our objective was to compare black and yellow pyramid traps baited with a lure to determine if one was superior for trapping herbivorous stink bugs. This study was conducted at four locations, three in Alabama and one in Georgia, over 2 yr. Additionally, residual efficacy of the lure was measured via trap capture over 1-mo intervals. Our results showed that only when native stink bug species were combined, and only in 1 yr, were captures significantly affected by trap color. Capture of the exotic H. halys and the most abundant native species, Euschistus servus (Say), was not significantly affected by trap color. Trap capture was significantly affected by how long a lure was in a trap. The data from this study suggests that when traps are used in conjunction with a pheromone to monitor multiple species of adult stink bugs, especially native species, the yellow pyramid trap is favored.
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