The aim of this study was to investigate the antibodies and DNA of Leptospira spp. isolated from infected cattle in a small rural dairy farm in a border region between Brazil and Paraguay. Blood and urine samples were collected from 50 Holstein cows aged between 1 and 15 years. The diagnostic tests performed were microscopic serum agglutination for antibody detection and polymerase chain reaction for Leptospira spp. detection. Out of the samples analyzed, 48% were MAT positive with titers ranging from 100 to 400, and the most prevalent antibody was to the serovar Hardjo. One serum sample was amplified to 549 bp for the sec y gene, and sequencing identified it as L. interrogans. This is the first report from northwestern Paraná (PR) State of L. interrogans identification in naturally infected milk cattle. Thus, based on these results, to enhance production efficiency, new serological and molecular studies on dairy cattle from border regions are required to characterize the epidemiology of possible genotypes and their consequences in affected herds.
A regulação e a auditoria são ferramentas de gestão presentes no Sistema Único de Saúde (SUS), que auxiliam na melhoria da qualidade dos serviços prestados. Todavia, para que haja melhoria na qualidade dos serviços prestados é necessário que as equipes que integram as centrais de regulação e auditoria sejam multiprofissionais. O presente estudo tem como objetivo evidenciar a importância da equipe multiprofissional nas centrais de regulação de auditoria do SUS. A literatura pesquisada evidenciou que é de relevada importância que as equipes de regulação e auditoria das centrais de regulação e auditoria do SUS sejam composta por equipes multiprofissionais qualificadas a fim de que as ações desenvolvidas por essas centrais seja de excelência para o fortalecimento da fortalecimento da gestão, dos serviços de saúde, da assistência ao usuário e da alocação dos recursos financeiros do SUS.
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