The present work was carried out in greenhouse conditions at the Centro de Investigación en Alimentación y Desarrollo AC in Delicias, Chihuahua, México. Four different concentrations (0, 25, 50 and 100 μM L) of Zn chelate and sulfate were used to study the antioxidant system of L. Three genes related with antioxidant activity [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] were selected for expression study. Results showed that when Zn chelate at 50 and 100 μM L were applied SOD was repressed and GSH-Px expression was low at 0, 25 and 100 μM L while with sulfate form SOD expression was low and GSH-Px expression was strong in all treatment. CAT was highly expressed in all form and treatments. For a biochemical study the same enzymes were spectrophotometrically measured. SOD activity shows differences in both forms of Zn, chelate form was different at 25, 50 and 100 μM L with less activity at 100 μM L and sulfate treatment shows differences in all concentrations used. GSH-Px activity shows significant differences with sulfate form at 25, 50 μM L where at 50 μM the activity was higher and low at 100 μM L, CAT does not exhibit significant differences but with chelate treatment at 50-100 μM L the activity was higher compared to sulfate. Finally, to raise the Zn concentration in bean under biofortification program is a promising strategy in cropping systems in order to increase the ingestion of zinc and antioxidant capacity in the general population and provided the benefits that this element offered in human health.
The present work was aimed to obtain a model for the determination of the leaf area in function of the length and width of the leaves in pepper (Capsicum annuum L.) hybrid Salvador. The research was carried out in nursery conditions at the Experimental Campus La Teodomira, located in the parish of Lodana, Santa Ana, province of Manabí, Ecuador, in 2016, in the stages of initiation of flowering and flowering-fructification. In each phase 100 physiologically mature leaves of different sizes were collected. Leaves were digitalized in a rectangle with known dimensions, which allowed the area to be calculated through the percentage of pixels of different colors. From the determination of the length and maximum width of each leaf and the estimated area through the digitization process, the regression models were obtained, selecting the better fit generated between the leaf area and the product of the length by the maximum width of the leaf ( ( ) = leaf area length * width f ). In the initiation of flowering stage the quadratic model generated the best coefficient of determination (R 2 = 0.958), whereas in the flowering-fructification stage the best coefficient of determination was achieved by the cubic model (R 2 = 0.955).The practical applicability of other simpler models among the tested ones, which show a high accuracy and sacrifice a low percentage of error, is discussed.
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