of Coccidioides immitis in monkeys. J. Bacteriol. 83:871-878. 1962.-Respiratory exposure to arthrospores from the submerged growth of Coccidioides immitis, strain Cash, in liquid medium resulted in similar pathogenesis in monkeys to that of strain Silveira arthrospores harvested from solid medium. Infectivity of 100% was noted with doses of 50 to 10,000 arthrospores. The disease was characterized by loss of appetite and weight, malaise, and extreme respiratory distress accompanied by coughing, with the immediate cause of death being acute coccidioidal pneumonia. The pathological picture was one of

Comparative lipid content, cell wall yield, neutral monosaccharide, glucosamine, and protein (amino acid) contents of arthrospores, mycelia, and spherules ofCoccidioides immitis Cash were studied. Cellular lipid contents were found in the decreasing order: spherules, arthrospores, mycelia. Lipid content of mycelia did not reach the level of arthrospores or spherules even when mycelia were grown on relatively rich media. Cell wall yields of spherules were lower than for mycelia when grown on comparable media. Cell walls of arthrospores, mycelia, spherules, and spherule culture filtrate all contained 3-0-methylmannose, mannose, and glucose, but in varying amounts. Cell wall yield and cell wall glucose content increased in mycelia grown in increasingly rich media, whereas mannose content either decreased or remained constant.

Documentation is offered for the identification of 3-0-methyl-mannose as one of several neutral sugars found in defatted arthrospore and mycelial cell walls of Coccidioides immitis. NOTESJ. BAcrERIOL.on August 5, 2020 by guest

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