<p>ABSTRACT</p><p>Phalaenopsis amabilis (L.) Blume and Phalaenopsis amboinensis J.J. Smith (diploid) are important in Phalaenopsis breeding. Polyploid species are needed for crossing with polyploid hybrid varities of Phalaenopsis. The objectives of this study were to obtain effective concentration of colchicine to induce polyploidy and to produce polyploid plantlets of P. amabilis and P. amboinensis. Experiment was arranged in randomized complete block design with one factor, the colchicine concentration. Protocorms of P. amabilis and P. amboinensis were immersed in half strength of Murashige-Skoog (1/2 MS) liquid media added with colchicine (0; 0.5; 5; 25; 50, and 75 mg L-1) for 10 days. The results showed that higher concentration of colchicine on both species did not have significant effect on the survival of the plantlets at 24 weeks after treatment. The average number of leaves and roots of colchicine treated planlets from both species were less than the control plantlets. Immersing protocorm in colchicine at concentration of 50 mg L-1 for 10 days was effective in inducing polyploid plantlets of P. amabilis and P. amboinensis with the frequency of 33.3% and 40%, respectively. Polyploid plantlet has larger stomata size and lower stomata density than the diploid ones.</p><p>Keywords: chromosome number, colchicine, polyploid, protocorm, stomatal density, stomatal size</p>

No abstract

This paper explores the role of Bogor Botanic Gardens (BBG) as a form of Nature-Based Solution (NBS) to mitigate Urban Heat Islands (UHI). Time series analysis of LANDSAT 8 OLI thermal band and Normalized Difference Vegetation Index (NDVI) was done from 2013 to 2020 using Google Earth Engine. Land Surface Temperature (LST) from Bogor and BBG were calculated, compared, and annual UHI areas were derived. The relationship of LST and NDVI were also explored annually to describe the effect of vegetation towards LST with linear regression. Overall, Bogor experiences a decrease of mean LST from 30.67°C and a maximum of 39.14°C in 2013 to 27.07°C and a maximum of 34.35°C in 2020. However, the inside of BBG is cooler with temperature ranging from 28.41°C and a maximum of 35.62°C in 2013 to 24.25°C and a maximum of 29.41°C in 2020. This is an effect of vegetation inside the BBG that regulate microclimate in its surrounding. It can be seen in the negative correlation between NDVI and LST observed with r2 ranging from 0.27 to 0.82. While UHI areas tended to increase from 8220 ha in 2013 to 8926 ha in 2020, BBG consistently acts as an urban cool island in the middle of UHI. Therefore, heat mitigation is proven to be one of the environmental services provided by BBG.

Abstract. Utama RS, Renyaan J, Nurdiansah D, Makatipu PC, Suyadi, Hapsari BW, Martha E, Rahayu EMD, Sugiharto A. Akbar N. 2022. Diversity of reef fish species in presence of mangrove habitat in Ternate, North Maluku, Indonesia. Biodiversitas 23: 5184-5193. Research on reef fish has been widely reported, where information about reef fish communities is an important factor for evaluating fisheries management and coral reef management. Mangroves as the nursery, foraging, and growing areas were also important to assure sustainable reef fish fisheries. This paper studies reef fish abundance and diversity in Ternate Waters in the presence of mangrove in coral reef habitats. To determines coral reef condition underwater photo transect was performed while an underwater visual census (UVC) was used to determine the abundance and diversity of reef fishes in Ternate waters between 2017 and 2018. A total of 14 stations were used, with eight stations near the mangroves and six stations that did not contain mangroves. Based on the observation, fish species richness in mangrove absence was higher than in the presence of mangroves, with 68 and 65 species in 2017 and 66 and 62 species in 2018. However, the abundance of reef fish was recorded high in the presence of mangroves than in the absence of mangroves, with 390 and 289 individual differences in 2017 and 2018. Mangroves' complexity affected part of reef fish communities in Ternate water, particularly Lutjanidae, Serranidae, and Scaridae, which might influence the abundance of reef fishes rather than the species richness. In addition, carnivore shows a negative effect in the presence of mangroves, in contrast with herbivores. It is related to mangrove functioning as a temporary shelter when high-pressure presence in their natural habitat (reef) and a place for foraging. Therefore, mangrove management must be a consideration in the coral reef or fisheries management program.

Abstract. Rahayu EMD, Yusri S. 2021. Habitat preferences of wild orchids in Bantimurung Bulusaraung National Park to model their suitable habitat in South Sulawesi, Indonesia. Biodiversitas 23: 43-54. Wild orchids are mainly threatened by habitat degradation and over-exploitation. Orchid conservation requires information regarding its distribution and suitable habitat, and factors affecting it. This study aimed to investigate the distribution and habitat preferences of orchids in Bantimurung Bulusaraung National Park, Sulawesi, Indonesia, and to predict the habitat suitability map of orchids in South Sulawesi, Indonesia. The survey was conducted in Bantimurung Bulusaraung National Park to collect data on orchid occurrences along with microhabitat characteristics (altitude, light, temperature, substrate thickness, and vertical distribution). ERGo Datasets Landforms and Physiography, SRTM elevation, NDVI derived from LANDSAT 8 OLI were used in Google Earth Engine to obtain macrohabitat data. Microhabitat preference of environmental data was averaged for each variable and further explored with Canonical Correlation Analysis. Suitable habitat was modeled with Maxent using occurrences and environmental data. The survey found 26 epiphytic orchid species and 22 phorophyte species. Orchids were found at an altitude from 514 m to 933 m above sea level. Altitude is the most discriminant factor in determining orchids’ distribution in the study area. The Maxent analysis showed that the suitable habitat of orchids comprises of 3,554.952 km2 area which is mainly located in lowland rainforest with high topographic diversity, preferably warm slope, and ridges. Since lower slope and lowland areas are not protected, community-based conservation such as social forestry can become one of the alternative solutions for in situ conservation of orchids. For ex-situ conservation, integrated orchid conservation in botanic gardens should be prioritized.