Mikroba telah banyak digunakan untuk meningkatkan hasil padi dengan System of Rice Intensification (SRI). Namun peningkatan produksi padi SRI menggunakan jamur Trichoderma spp. indigenous belum diketahui. Penelitian ini bertujuan untuk mengevaluasi pengaruh isolat jamur Trichoderma spp. indigenous yang diisolasi dari berbagai varietas padi terhadap peningkatan hasil padi varietas Junjuang dengan metode SRI. Penelitian ini menggunakan Rancangan Acak Lengkap dengan enam jenis isolat Trichoderma spp. indigenous, satu kontrol dan tiga ulangan. Perlakuan: T0 = Kontrol (tanpa isolat Trichoderma spp.), T1 sampai T6 berturutturut adalah isolat jamur Trichoderma spp. indigenous, diekstrak dari rhizosfer padi varietas Kuning Kurik, Pandan Wangi, Junjuang, Silih Baganti, Ketan Merah, dan Sokan. Hasil penelitian menunjukkan bahwa aplikasi isolat jamur Trichoderma spp. indigenous nyata meningkatkan tinggi tanaman dan jumlah anakan padi dibandingkan dengan kontrol. Aplikasi isolat Trichoderma spp. indigenous meningkatkan jumlah gabah per malai antara 52 sampai 110% dibandingkan T0 dan tertinggi pada T3. Persentase gabah hampa terrendah pada T3 (5%) dan tertinggi pada T0 adalah (32%). Berat gabah kering per pot tertinggi pada T3 (98,2 g) dan nyata lebih tinggi dibandingkan pada T0 (58,4 g). Berat 1.000 biji tertinggi pada T3 (20,7 g) dan terendah pada T0 (17,9 g). Penelitian ini menyimpulkan bahwa isolat Trichoderma spp. indigenous dari rhizosfer varietas Junjuang (T3) memberikan pengaruh tertinggi terhadap peningkatan hasil padi varietas Junjuang dengan metode SRI. Terdapat kesesuaian isolat Trichoderma spp. indigenous dari rhizosfer varietas Junjuang dengan tanaman inangnya sehingga isolat tersebut meningkatkan pertumbuhan dan produksi tanaman.
Differences in location and rhizosphere cause differences in species diversity and population of Arbuscular Mycorrhizal Fungi (AMF). In addition, not all AMF have the same morphological and physiological characteristics, therefore it is very important to know their identity. This study aims to determine the presence and number of spores as well as to determine the morphological characteristics of AMF originating from the rhizosphere of several horticultural crops in the agricultural land of Payakumbuh State Agricultural Polytechnic. The method used in this research is descriptive exploratory method by means of purposive sampling for soil sampling. While the stages of this research include: taking and collecting data in the field, determining the point of location for soil sampling, taking soil samples, analyzing soil properties in the laboratory, isolating AMF spores and identifying AMF spores morphologically. The conclusions of this study are 1) The population of AMF spores in horticultural land is high. The highest spore population was found in soil samples of the root area of shallot plants (556 spores per 10 g of soil), while the lowest number of spores was in soil samples of eggplant root areas (271 spores per 10 g of soil), 2) AMF exploration in several horticultural crops in the agricultural land of the Payakumbuh State Agricultural Polytechnic, based on morphological identification (shape, color and size), the AMF found consisted of three genera, namely Glomus sp, Gigaspora sp, and Scutelospora sp.
Arbuscular Mycorrhizal Fungi (AMF) of indigenous believed to be more efficient and effective in helping the growth of plants. Observations diversity of indigenous AMF conducted at several locations of centers of shallot cultivation in West Sumatra. AMF existence is closely related to environmental factors (abiotic and biotic). Sampling was based on the different in altitude : highland (Alahan Panjang), middle land (Saniang Baka) and lowland (Kambang). Indigenous AMF identification using morphological approach. The present study was undertaken to 1) look at the population and species diversity of AMF indigenous derived from shallots rhizosphere in different altitudes in West Sumatra and determine whether there are differences on the number and type of AMF at any location, 2) identified morphological characters of AMF spores. 19 types of indigenous AMF derived from shallots cropland in West Sumatra that Scutelospora (4 types), Glomus (10 types), Gigaspora (2 types), Acaulospora (3 types). We found the genus Glomus dominant in this study. Of the three locations, the greatest of AMF types was found in the area Saniang Baka (13 species), followed by Alahan Panjang (12 types) and Kambang (10 types). The number of species did not always a correlate with the number of spores. AMF spore count decreased with increasing height above sea level. Differences in altitude correlated negatively with the number of spores produced. The lowland, Kambang having a higher spore count (817 spores) than Saniang Baka in the middle land (798 spores) and Alahan Panjang in the highland (687 spores). It appears that differences in altitude affect the production of spores.
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