Dental health education conducted to the elementary school students is one of the promotive efforts to increase oral and dental health, however, it needs an appropriate media. This study was aimed to evaluate the difference between health promotion using audio media and using audio-visual media to dental health behavior of elementary school students. This was a quasy experimental study using questionnaire as the instrument. This study was conducted among elementary school students of Sekolah Dasar Inpres at Tiwoho North Minahasa. There were 56 students as respondents obtained by using simple random sampling method. The result of independent T-test showed a difference between the average behavior value of the audio group and of the audio-visual group (p= 0.004). Conclusion: There was a significant difference between health promotion using audio media and using audio-visual media to care dental health behavior among elementary school students. Keywords: health promotion, audio, audio-visual Abstrak: Promosi kesehatan gigi dan mulut pada siswa sekolah dasar merupakan salah satu upaya promotif dalam meningkatkan kesehatan gigi dan mulut anak. Untuk melakukan hal tersebut diperlukan penggunaan media yang tepat. Penelitian ini bertujuan untuk mengevaluasi perbandingan pengaruh promosi kesehatan menggunakan media audio dengan media audio-visual terhadap perilaku kesehatan gigi dan mulut pada siswa SD. Penelitian dilakukan di SD Inpres Tiwoho Minahasa Utara, dengan jenis penelitian quasy eksperimental menggunakan kuesioner. Jumlah responden sebanyak 56 siswa diperoleh dengan teknik simple random sampling. Hasil uji statistik independent T-test menunjukkan perbedaan antara nilai rerata kelompok audio-visual dan kelompok audio (p=0,004). Simpulan: Terdapat perbedaan bermakna antara promosi kesehatan menggunakan media audio dengan media audio-visual terhadap perilaku pemeliharaan kesehatan gigi dan mulut pada siswa SD.Kata kunci: promosi kesehatan, audio, audio-visual