Chronic Kidney Disease is evident if the blood urea level is more than 200 mg/dl. Uremia causes a malfunction in almost all organ systems such as; fluid and electrolyte disorders, metabolic endocrine, neuromuscular, cardiovascular and pulmonary, skin, gastrointestinal, hematological, and immunological. Hemodialysis is an attempt to reduce the symptoms of uremia so that the patient's clinical condition can also improve. The purpose of this study was to determine the description of Ureum and Creatinine in CKD Patients undergoing Hemodialysis. This type of research is observational descriptive. The sample included all CKD patients undergoing hemodialysis at the Ir Sukarno Sukoharjo Regional Hospital in 2019 who met the inclusion criteria, so as many as 83 samples were obtained. The type of data is secondary data obtained from medical records. The results showed that most patients were in the 40-60 years age group of 72% and the majority of the male sex were 51 patients (61%). Urea and creatinine appearance in patients undergoing hemodialysis has increased very high. In 83 patients with CKD increased serum creatinine levels ( 100%) with a mean creatinine level in men of 11.80 mg / dL and women of 9.73 mg / dL and an increase in ureum levels with a mean of 167 men, 09 mg / dL and women of 164.39 mg / dL. This study concludes that all patients with CKD have increased levels of urea and creatinine by more than 100%.

Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol.

Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. xylene is the most commonly used aromatic solvent for clearing agents and deparaffinizing agents in histopathology laboratories, but xylene is one of the most dangerous chemicals found in histology laboratories. Based on the dangers posed by xylene, a replacement material is needed. Several xylene substitutes such as reagents derived from limonene, aliphatic hydrocarbons, vegetable oils and mineral oils have been commercially developed, but the available xylene substitutes are still less effective, more expensive, and still as dangerous as xylene itself. Substitutes that are considered safer are from natural oils, one of which is olive oil which has similarities with xylene, namely in hydrocarbon and phenol compounds. This study used two types of tissue, namely skin and liver from mice (Mus musculus) which were cut into two parts; the first part uses xylene as and the other part uses olive oil as a clearing agent. The assessment of hematoxylin eosin staining results were determined based on the category of assessment of cell structure, such as the cell nucleus, cytoplasm, and color uniformity carried out by three readers and five fields of view for each microscopic slide at 40X magnification of the objective lens. Comparison of observations of the xylene group in skin and liver tissue 100% got a good score on the cell nucleus, cytoplasm, and color uniformity. (p=1,000). The olive oil group had a slight difference in color uniformity in liver tissue when compared to skin, but not statistically different (p=0.773). The comparison of the overall readings of the xylene and olive oil groups on skin and liver tissue also showed no statistically different (p=0.262). So it can be concluded that olive oil can be recommended as a substitute for xylene in the clearing process in histological tissue processing.

Introduction: Monosodium Glutamate (MSG) increase free radicals and effecting on male reproductive hormone balance. CMCE propolis extract can be used as supplement to protect cells from free radical.Objective : To prove the effect of CMCE propolis extract to increase FSH, LH and Testosterone levels in male Wistar rats induced by MSG.Methods : This research was an experimental with post test only control group design used 18 male Wistar rats and divided into 3 groups. Each group on Control (K), P1 and P2 were induced by MSG of 140mg, then on P1 added 8,3mg CMCE propolis extract and P2 10,8mg for 21 days. Then on 22th day of blood was taken through the orbitalis sinus and serum was used for the analysis FSH, LH and Testosteron levels with ELISA method.Results : ANOVA analysis test showed significant differences each group p=0,000(p˂0,05). Post hoc LSD test showed FSH, LH and Testosterone levels in the 8,3mg dose CMCE propolis extract and 10,8mg were significant different compared with control group p=0,000(p˂0,05). CMCE propolis extract 10,8mg dose had the significantly highest effect of FSH, LH and Testosterone levels than CMCE propolis extract 8,3 mg.Conclusion : CMCE propolis extract 10,8mg/day for 21 days is proven to increase FSH, LH and testosterone levels in male Wistar rats induced by MSG.

Honey and cinnamon are plant products that have many properties, one of which is used as an antibacterial agent for various diseases. The effectiveness of the two active substances against bacteria in the Enterobacteriaeae family is unclear. The study was conducted to determine the differences in the effectiveness of forest honey and cinnamon in inhibiting the growth of bacteria in the Enterobacteriaceae family. This research method is an experimental study with a combination method of diffusion and wells using samples of the bacteria Klebsiella pneumoniae, Esherichia coli, Salmonella typhi, Serratia marcescens, and Enterobacter cloacae. Forest pure honey and cinnamon were prepared as test solutions with concentrations of 25%, 40%, 55%, 70%, 85% and 100%. The results obtained were the inhibition zone formed on Nutrient Agar Plate (NAP) media, in forest pure honey the inhibition zone formed in S. typhi, E. coli, Klebsiella pneumoniae, Serratia marcescens, and Enterobacter cloacae was greater at 100% concentration, namely 30.5mm, 32.5mm, 38mm, 29.5mm, 26mm, while the 100% concentration of cinnamon showed results, namely 12mm, 10mm, 8.5mm, 11mm, and 11mm. Conclusion is Forest pure honey was more effective at inhibiting bacteria from the Enterobacteriaceae family than cinnamon.