We examined the responsiveness of large luteal cells (LLC), small luteal cells (SLC), and endothelial cells of the Day 4 and Day 10 bovine corpus luteum (CL) to prostaglandin (PG) F2alpha and endothelin (ET)-1. Using a single-cell approach, we tested the ability of each agonist to increase the cytoplasmic concentration of calcium ions ([Ca2+]i) as function of luteal development. All tested concentrations of agonists significantly (P = 0.05) increased [Ca2+]i in all cell populations isolated from Day 4 and Day 10 CL. Day 10 steroidogenic cells were more responsive than Day 4 cells to PGF2alpha and ET-1. Response amplitudes and number of responding cells were affected significantly by agonist concentration, luteal development, and cell type. Response amplitudes were greater in LLC than in SLC; responses of maximal amplitude were elicited with lower agonist concentrations in Day 10 cells than in Day 4 cells. Furthermore, on Day 10, as the concentration of PGF2alpha increased, larger percentages of SLC responded. Endothelial cells responded maximally, regardless of agonist concentration and luteal development. In experiment 2, we tested the developmental responsiveness of total dispersed and steroidogenic-enriched cells to the inhibitory actions of PGF2alpha and ET-1 on basal and LH-stimulated progesterone accumulation. The potency of PGF2alpha steroidogenic-enriched cells on Day 4 was lower than on Day 10; in contrast, the potency of ET-1 was not different. Therefore, ET-1 was a tonic inhibitor of progesterone accumulation rather than a mediator of PGF2alpha action. The lower efficacy of PGF2alpha in the early CL more likely is related to signal transduction differences associated with its receptor at these two developmental stages than to the inability of PGF2alpha to up-regulate ET-1.

A single-cell approach for measuring the concentration of cytoplasmic calcium ions ([Ca(2+)](i)) and a protein kinase C-epsilon (PKCepsilon)-specific inhibitor were used to investigate the developmental role of PKCepsilon in the prostaglandin F(2alpha)(PGF(2alpha))-induced rise in [Ca(2+)](i) and the induced decline in progesterone accumulation in cultures of cells isolated from the bovine corpus luteum. PGF(2alpha) increased [Ca(2+)](i) in Day 4 large luteal cells (LLCs), but the response was significantly lower than in Day 10 LLCs (4.3 +/- 0.6, n = 116 vs. 21.3 +/- 2.3, n = 110). Similarly, the fold increase in the PGF(2alpha)-induced rise in [Ca(2+)](i) in Day 4 small luteal cells (SLCs) was lower than in Day 10 SLCs (1.6 +/- 0.2, n = 198 vs. 2.7 +/- 0.1, n = 95). A PKCepsilon inhibitor reduced the PGF(2alpha)-elicited calcium responses in both Day 10 LLCs and SLCs to 3.5 +/- 0.3 (n = 217) and 1.3 +/- 0.1 (n = 205), respectively. PGF(2alpha) inhibited LH-stimulated progesterone (P(4)) accumulation only in the incubation medium of Day 10 luteal cells. Both conventional and PKCepsilon-specific inhibitors reversed the ability of PGF(2alpha) to decrease LH-stimulated P(4) accumulation, and the PKCepsilon inhibitor was more effective at this than the conventional PKC inhibitor. In conclusion, the evidence indicates that PKCepsilon, an isozyme expressed in corpora lutea with acquired PGF(2alpha) luteolytic capacity, has a regulatory role in the PGF(2alpha)-induced Ca(2+) signaling in luteal steroidogenic cells, and that this in turn may have consequences (at least in part) on the ability of PGF(2alpha) to inhibit LH-stimulated P(4) synthesis at this developmental stage.

Sexual violence is a serious public health problem that has been associated with negative mental and physical health outcomes. Few existing studies have examined the prevalence and patterns of adverse mental health among victims of sexual violence using data from nationally representative samples of U.S. adults. The main objectives of this study were to identify patterns in the associations between sexual violence victimization and depression and anxiety (DA) symptoms using data from the sexual violence and DA Behavioral Risk Factor Surveillance System (BRFSS) modules. Stratified multivariate logistic regression models were conducted to test the associations between sexual violence victimization and DA controlling for demographic characteristics. Multiple stratified MANOVA models were used to detect the effect of sexual violence victimization on DA symptoms while controlling for key demographic characteristics. Among all 61,187 participants, more than 5% (n = 3,240) were victims of sexual violence, out of which 18.82% reported being diagnosed with depression, 8.37% reported an anxiety disorder, and 28.28% reported being diagnosed with DA disorder. Victims of sexual violence reported significantly higher number of days when they had trouble concentrating, sleep difficulties, poor appetite, little interest or pleasure in activities, blamed themselves for personal failure, felt depressed, and had little energy. The present study highlights the importance of collecting nationally representative data from victims of sexual violence and extends previous findings from clinically based studies. This study also serves as an example of an analytic approach that addresses a public health priority area by drawing on data from multiple topic-specific BRFSS modules.

In the United States, an estimated three million men are victims of sexual violence each year, yet the majority of existing studies have evaluated the consequences and characteristics of victimization among women alone. The result has been a gap in the existing literature examining the physical and psychological consequences of sexual assault among men. The main objective of this study was to identify health outcomes, risk behaviors, and perpetrator/victim relationship characteristics among men who have experienced an attempted or completed sexual assault using data from the sexual violence module of the Behavioral Risk Factor Surveillance System survey. A total of 59,511 male respondents participated in the sexual violence module, and the majority of participants were White (73.7%), between the ages of 35 to 44 years (19.8%), married (69.0%), graduated from college (34.6%), and had an annual household income of more than US$50,000 (49.9%). Stratified multivariate logistic regression models were conducted to test the associations between victimization and health outcomes and risk behaviors controlling for age, marital status, race/ethnicity, income, education, and other potential confounders. Results of these analyses suggest important associations between health and sexual violence victimization. Specifically, men who reported unwanted attempted intercourse and attempted and completed intercourse were more likely to report poor mental health, poor life satisfaction, activity limitations, and lower emotional and social support. The current study extends knowledge of consequences of male sexual violence by considering characteristics of sexual assault and by identifying associations between victimization and a broad range of health indicators.

An estimated 1 out of 6 women and 1 out of 33 men has been a victim of sexual assault at some point in their lifetime. The objective of this study was to quantify the associations between sexual assault and negative health outcomes among males and females who reported being sexually assaulted in the past 12 months or at some point before the past 12 months. Data were obtained from the 2005 Behavioral Risk Factor Surveillance System core and sexual violence modules. A series of logistic regression models, including all respondents and stratified by gender, was used to identify differences associated with victimization. Among women, victimization before the past 12 months was significantly associated with poor health status, poor mental health, lower life satisfaction, activity limitations, smoking, and binge drinking. Women who reported victimization in the past 12 months were also significantly more likely to report poor mental health, lower life satisfaction, activity limitations, and binge drinking. Among males, significant associations were found with smoking (past 12 months), poor life satisfaction (before the past 12 months) and activity limitations (before the past 12 months). Results of this study suggest that poor mental and physical health associated with victimization are more prevalent in women and that these relationships persist over time. The broad range of outcomes associated with victimization suggests that further research is needed to better understand the consequences associated with sexual violence across the lifespan.