Keywordsklebsiella species are one of the key issues that have been steadily rising in intensive poultry production, causing great economic losses. The main cause of this uncomfortable condition is the increasing resistance of bacteria to antibiotics and has food safety risks because it can act as a source of contamination for chicken meat and eggs. In the present study, we aim to isolation and identification of klebsiella spp. and Molecular screening of antibiotics resistance genes that present in isolated Klebsiella spp. Twenty-Eight klebsiella species obtained from 50 healthy chickens differentiated into 21/28 (75%) Klebsiella pneumoniae and 7/28 (25%) Klebsiella oxytoca. Antimicrobial sensitivity testing against 12 commonly used antibiotics in chicken farms revealed that klebsiella species were fully resistant to oxytetracycline (100%) and penicillin (100%) and trimethoprim (100%) and moderate resistance to cefadroxil, doxycycline, amoxicillin/clavulanic acid, chloramphenicol, cefotaxime and lowest resistance to meropenem and klebsiella species extremely susceptible to amikacin (100%), tobramycin (100%) and norfloxacin (100%). A total of 12 klebsiella species differentiated into 9 klebsiella pneumoniae and 3 klebsiella oxytoca screened to find ESBL coding gene in the klebsiella species. The isolates were found to have bla SHV (100%), bla TEM (91.7%), and bla CTX-M (83.4%).