Molecular studies on some antibiotic-resistant genes of Klebsiella species isolated from chicken

Abstract

Keywordsklebsiella species are one of the key issues that have been steadily rising in intensive poultry production, causing great economic losses. The main cause of this uncomfortable condition is the increasing resistance of bacteria to antibiotics and has food safety risks because it can act as a source of contamination for chicken meat and eggs. In the present study, we aim to isolation and identification of klebsiella spp. and Molecular screening of antibiotics resistance genes that present in isolated Klebsiella spp. Twenty-Eight klebsiella species obtained from 50 healthy chickens differentiated into 21/28 (75%) Klebsiella pneumoniae and 7/28 (25%) Klebsiella oxytoca. Antimicrobial sensitivity testing against 12 commonly used antibiotics in chicken farms revealed that klebsiella species were fully resistant to oxytetracycline (100%) and penicillin (100%) and trimethoprim (100%) and moderate resistance to cefadroxil, doxycycline, amoxicillin/clavulanic acid, chloramphenicol, cefotaxime and lowest resistance to meropenem and klebsiella species extremely susceptible to amikacin (100%), tobramycin (100%) and norfloxacin (100%). A total of 12 klebsiella species differentiated into 9 klebsiella pneumoniae and 3 klebsiella oxytoca screened to find ESBL coding gene in the klebsiella species. The isolates were found to have bla SHV (100%), bla TEM (91.7%), and bla CTX-M (83.4%).

Antimicrobial Resistance and Virulence-Associated Genes of Aer-Omonads Isolated From Lake Manzala Water and Wild Nile Tilapia: Implications to Public Health and the Lake Microbial Community

“…A specific amplification band of the tetA(A) gene was detected in all Aeromonas isolates tested, which agrees with the findings from another study (46). The streptomycin resistance gene, aadA1, was detected in only one strain of A. hydrophila (6.2%) from O. niloticus, which is less than that reported in other studies (30, 47).…”

Molecular Studies on Some Virulent and Multi-Drug Resistant Cattle Klebsiella Strains and their Heamatobiochemical Impacts

“…ESBL bacteria could produce extendedspectrum β-lactamases enzymes, making them survive longer than other Gram -ve bacteria in the environment. MDR ESBL K. pneumoniae strains could put human and animal health at risk since it limited the therapeutic choices prompting the usage of colistin antibiotic, which was no longer utilized owing to toxicity (Abdeltawab et al, 2022). ESBL strains could harbour (bla CTX-M , bla TEM and bla SHV ) genes.…”

“…Moreover, ESBL genes could be mutated, creating novel enzymes with extended substrate proles. Indeed, over 300 distinct ESBL genes were recorded, which had been classified according to their amino acid sequence into nine different structural and evolutionary groups (Abdeltawab et al, 2022).…”

Antibacterial and antibiofilm effect of nano zinc-oxide and propolis nanoemulsion against strong biofilm producer coliform species isolated from chickens

“…It includes Escherichia coli, Klebsiella, Enterobacter and Citrobacter (Markey et al, 2013). The most pathogenic member of coliform is E. coli that causes many diseases such as colisepticemia, coligranuloma, swollen head syndrome, meningitis, air sacculitis, and pericarditis in both humans and animals (Fairbrother et al, 2005).Bacteria attached each other forming biofilm which protect it from antimicrobial agents causing high economic losses (Chakraborty et al, 2018). Genus Klebsiella has many species as K. pneumoniae, K. oxytoca, K. ozanae and K. rhinoscleromatis.…”