As a result of the rapid increase in the world population, the need for wheat, which is one of the main nutrition in the human diet, is also rapidly increases. However, due to yield losses caused by abiotic stress factors such as drought, wheat production is not sufficient. Therefore, genetic characterization studies performed on wheat genotypes in terms of drought tolerance are important. In this study, genetic characterization of wheat genotypes regardingdrought tolerance was carried out by using molecular markers associated with drought-tolerance genes. For this purpose, 14 polymorphic markers were used to be able to distinguish between the control groups. Genetic characterization of 27 bread wheat genotypes by using eight ISSR markers revealed a polymorphism rate of 75.8%, and the mean PIC was calculated as 0.55. Based on the results of the genetic characterization performed with six SSR markers, the mean PIC value was 0.77, the mean He was 0.79, and the mean allele number was 6.7. In this study, the characterization of drought-tolerant and sensitive genotypes was carried out, and the potentials of genotypes for breeding studies were revealed. This study also indicates that used SSRs and ISSRs markers are useful in marker-assisted breeding about drought tolerance.