The study presents the preparation and characterization of new scaffolds based on bacterial cellulose and keratin hydrogel which were seeded with adipose stem cells. The bacterial cellulose was obtained by developing an Acetobacter xylinum culture and was visualized using SEM (scanning electron microscopy) and elementally determined through EDAX (dispersive X-ray analysis) tests. Keratin species (β–keratose and γ-keratose) was extracted by hydrolytic degradation from non-dyed human hair. SEM, EDAX and conductometric titration tests were performed for physical–chemical and morphological evaluation. Cytocompatibility tests performed in vitro confirmed the material non-toxic effect on cells. The scaffolds, with and without stem cells, were grafted on the burned wounds on the rabbit’s dorsal region and the grafts were monitored for 21 days after the application on the wounds. The clinical monitoring of the grafts and the histopathological examination demonstrated the regenerative potential of the bacterial cellulose–keratin scaffolds, under the test conditions.

Computed tomography (CT) is a most valuable technique for the diagnosis of abdominal disorders. In veterinary medicine, x-ray and ultrasound are the imaging modalities of choice for the abdomen, but CT can provide information that cannot be obtained by other means. One mature, female, domestic cat, 4 kg weight was used for this study. The cat was clinically healthy, with no abnormal findings at hematological and biochemical analysis. It was premedicated with 20 µg/kg medetomidine and 0.3mg/kg butorphanol IM. For induction of anesthesia, 3 to 5 mg/kg propofol was administered IV. Anesthesia was maintained with a mixture of isoflurane and oxygen. The patient was positioned in sternal recumbency. CT scans were performed with a helical CT scanner Hi Speed CT/e Dual; General Electric. Technical factors were 120 kVp and 50 mA settings, with 1 rotation/s. All sections were imaged with soft-tissue window. CT scans were performed after IV injection of iodinated contrast medium, iopamidol, 2 mL/kg. The images are presented in progression from the 8th thoracic vertebra to the level of the pelvic inlet. Identifiable anatomical structures are labelled on the CT images. The stomach is left-sided with the gastric folds less numerous and thinner than those in the dogs. The normal small intestine contains less gas in cats. The feline cecum is very short. The liver showed a low attenuation with the mediastinum-vascular window settings, allowing better visualisation of the hepatic lobes and interlobular fissures. The gallbladder and bile ducts were also better visualised. CT imaging studies can provide additional information in patients with radiographic and ultrasonographic patterns of abdominal disorders, as a specialised imaging method, a useful preoperative technique and a precise staging tool. CT improves the image quality further and allows better timing of vascular phases. Helical CT angiography enables visualization of contrast-enhanced abdominal vessels using only a peripheral venous injection of iodinated contrast medium.

76 cats were clinical examined for mammary and abdominal neoplasias. In 41 cats were performed microscopic examination. Regarding the location of the primary tumors 70 cats had mammary tumors. Isolated, six cases of primary non-mammary tumors were diagnosed following necropsy and histopathology. Following cytopathological and histopathological examinations, 14 tumor types were identified, of which 3 benign and 11 malignant. Benign tumoral types consisted of lipoma, vesical leiomyoma and mammary adenoma. Diagnosed malignant cases consisted of simple mammary adenocarcinoma, solid adenocarcinoma, compact adenocarcinoma, hepatic cholangiocarcinoma, compact carcinoma, mixed pulmonar bronchioloalveolar carcinoma, hemangiosarcoma, mammary comedocarcinoma, solid carcinoma and mixed adenocarcinoma.

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