Context:The fluoride release of sealants in vitro shows a marked decrease. Giomers are distinguishable from manufactured resin-based sealants and contain prereacted glass-ionomer particles (PRG).Aims:To compare the amounts of fluoride released from the main pit and fissure of a resin-based sealant with that from a Giomer and to assess the abilities of the sealant and the Giomer to recharge when exposed to regular use of fluoride rinse.Materials and Methods:The readings for the fluoride concentration were carried out for 60 days using a fluoride ion-specific electrode. After this period, the samples were recharged using a fluoride mouth rinse. The amount of fluoride released after this recharge was determined for 5 days. The data were analyzed using Student's t- and analysis of variance tests.Results:In general, all materials presented higher fluoride release in the first 24 h; G1 and G4 showed a higher fluoride release in this period. On the other hand, G3 and G1 presented the most constant fluoride release until the 8th day, wherein all the sealants considerably decreased in the amount of fluoride released.Conclusion:G1 and G3 released higher concentrations of fluoride, although no significant differences were found. Giomers recharged in the first 24 h after polymerization presented an improved and sustained fluoride release.
Syzygium aromaticum (clove) has been used as a dental analgesic, an anesthetic, and a bioreducing and capping agent in the formation of metallic nanoparticles. The main objective of this study was to evaluate the antimicrobial effect in oral microorganisms of biogenic silver nanoparticles (AgNPs) formed with aqueous extract of clove through an ecofriendly method “green synthesis”. The obtained AgNPs were characterized by UV-Vis (ultraviolet-visible spectroscopy), SEM-EDS (scanning electron microscopy–energy dispersive X-ray spectroscopy), TEM (transmission electron microscopy), and ζ potential, while its antimicrobial effect was corroborated against oral Gram-positive and Gram-negative microorganisms, as well as yeast that is commonly present in the oral cavity. The AgNPs showed absorption at 400–500 nm in the UV-Vis spectrum, had an average size of 4–16 nm as observed by the high-resolution transmission electron microscopy (HR-TEM), and were of a crystalline nature and quasi-spherical form. The antimicrobial susceptibility test showed inhibition zones of 2–4 mm in diameter. Our results suggest that AgNPs synthesized with clove can be used as effective growth inhibitors in several oral microorganisms.
Dental demineralization was determined in patients at three time points during fixed orthodontic treatment. A multiple cross-sectional study included 108 patients divided into three different groups: (1) beginning of orthodontic treatment; (2) one year into treatment; and (3) two years into treatment. Demineralization was estimated using a DIAGNOdent pen. We obtained data from multiple tooth-by-tooth demineralization readings combined with salivary pH and patients’ oral hygienic and dietary behaviors. A t-test for independent samples and Spearman´s correlation were performed. No demineralizations differences were found between the initial stage and one year into treatment. Between one and two years small differences were observed, but demineralization increased between the initial stage and second treatment year, predominating in upper right central incisors (p = 0.056), upper left lateral incisors (p = 0.040), both upper canines (p = 0.055 and p = 0.040, respectively) and first left premolars (p = 0.034 and p = 0.053, respectively). We did not find associations between oral hygiene and dietary behaviours or salivary pH. In conclusion, demineralization occurred in first year of treatment and increased during second year, predominating in the upper arch and the left side mainly in upper right central incisors, upper left lateral incisors, both upper canines, and first left premolars.
Background: Several efforts have been made to improve the glass ionomer cements (GICs) properties with nanotechnology. Fluoride release in once of most beneficial properties of GICs. The purpose of this study was to evaluate the fluoride release, recharge, and cytotoxicity in GICs reinforced with titanium dioxide nanoparticles (TiO 2 N).Objective: Evaluate the fluoride release, recharge, and cytotoxicity in GICs reinforced with TiO 2 N.Methods: Four GICs, FUJI IX EXTRA (G1c), KETAC MOLAR (G2c), IONOFILL MOLAR (G3c), and FUJI IX (G4c) were combined with TiO 2 N (G1e, G2e, G3e, and G4e) and divided into blocks of 5-mm width and 1-mm thickness 10 each. A total of 80 samples were arranged as follows: GICs alone as negative control (n = 40) and GICs + TiO 2 N as experimental groups (n = 40). The fluoride release was determined for periods of 1, 2, 6, 10, 31, 90, 180, 240, and 300 days. On days 30 and 179, samples were recharged by submerging in 1 mL of 20,000 ppm sodium fluoride gel. Cytotoxic activity was carried out with gingival fibroblasts, using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide cell viability assay.Results: The experimental groups obtained the highest and more constant fluoride released when compared to control groups. After the first recharge, experimental groups (G1e, G3e, and G4e) showed statistically significant results (P = .001, 0.010, and 0.001 respectively) enhancing their recharge ability regarding control groups. The second recharge showed better results in G1e concerning the rest of the groups. No cytotoxic activity was observed in all experimental groups, although significant differences were observed in G3e and G4e regarding control group. Conclusion:The incorporation of TiO 2 N enhance the fluoride release in glass ionomers with a noncytotoxic effect on human gingival fibroblasts.Abbreviations: GICs = glass ionomer cements, G1c = Glass ionomer FUJI IX EXTRA, G2c = Glass ionomer KETAC MOLAR, G3c = Glass ionomer IONOFILL MOLAR, G4c = Glass ionomer IONOFILL MOLAR, G1e = Glass ionomer FUJI IX EXTRA modified with titanium dioxide nanoparticles, G2e = Glass ionomer KETAC MOLAR modified with titanium dioxide nanoparticles, G3e = Glass ionomer IONOFILL MOLAR modified with titanium dioxide nanoparticles, G4e = Glass ionomer IONOFILL MOLAR modified with titanium dioxide nanoparticles,HGF = human gingival fibroblasts, PDL = population doubled level, TiO 2 = titanium dioxide, TISAB = total ionic strength adjustment buffer solution, TiO 2 N = titanium dioxide nanoparticles.
Se presentan los aspectos clínicos, anatomopatológicos e inmunohistoquímica de un caso de COCE diagnosticado tardíamente con desenlace fatal, así como un reporte retrospectivo-descriptivo. Se analizan los datos de la historia clínica, el estudio anatomopatológico y se mide el índice de proliferación celular a través del anticuerpo Ki-67. El resultado de este caso en etapa avanzada de cáncer es la muerte del paciente. Se resalta la importancia de un examen bucal realizado por un profesional con capacidad de identificar lesiones de forma precoz que pueda contribuir en la disminución de muertes por COCE en México. El carcinoma oral de células escamosas (COCE), que afecta en especial a hombres mayores de 50 años, es causado principalmente por el consumo excesivo de alcohol y tabaco.
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