No abstract

The purpose of this work was to study the genetic determinants responsible for extended-spectrum cephalosporin (ESC) resistance of Salmonella collected during the period of 1995-2008 at the Hussein Dey hospital in Algiers (Algeria). Fourteen ESC-resistant Salmonella isolates were tested towards 22 antimicrobial agents. Polymerase chain reaction (PCR) and sequencing were used to determine the underlying genetic determinants responsible for the extended-spectrum beta-lactamase (ESBL) phenotypes. Enterobacterial Repetitive Intergenic Consensus PCR was employed to type the isolates. All tested isolates were resistant to ticarcillin, ticarcillin-clavulanate, piperacillin, cefuroxime, aztreonam, ceftazidime, cefotaxime (except two isolates), cefepime, and cefpirome. PCR and DNA sequencing identified these ESBLs as TEM-48 (n=6), TEM-4 (n=3), CTX-M-15 (n=4), and one new TEM, designated TEM-188. Thus, continued surveillance for the presence of ESBL-producing (non-typhoidal) salmonellae in Algeria is essential.

Abstract:A new cycloheptapeptide named akeassimezorine 1 and twelve known compounds: methoxychelerythrine 2, chelerythrine 3, nitidine 4, methoxyfagaridine 5, jatrorrhizine 6, tembetarine 7, N-methylatanine 8, magnoflorine 9, skimmianine 10, sesamine 11, hesperidine 12, and lupeol 13 were isolated from the roots of Zanthoxylum mezoneurispinosum Aké Assi (Rutaceae). The structures of those compounds were elucidated on the basis of one-and two-dimensional NMR and HR-ESI-MS analyses. Antibacterial activity of compound 1 was evaluated against pathogenic agents. This study constituted the first phytochemical investigation of the roots of Zanthoxylum mezoneurispinosum.

No abstract

Hemolytic-uremic syndrome due to enterohemorrhagic Escherichia coli, belonging to serogroup O91 has rarely been described. We report here a case of post-diarrheal HUS due to EHEC O91 in an elderly patient for whom diagnosis was delayed given a previously diagnosed C. difficile infection. This case highlights the usefulness of Shiga-toxin detection.