The aim of this study was to qualitatively investigate the effects of a zinc-carbonate hydroxyapatite (Zn-CHA) containing toothpaste on stripped enamel morphology in a pH cycling model in vitro and to compare the efficacy of this toothpaste versus fluoride one which still represent the gold standard to remineralize early enamel lesions. Twenty-one extracted lower incisors underwent to interproximal enamel reduction with metal strips (Horico 80 µm) on both mesial and distal surfaces. They were then sliced into mesial and distal halves and the 42 samples obtained were randomly assigned to 3 groups of 14 enamel specimens each. For 8 days, teeth were placed in lactic acid solution for 2 h three times a day with 2 h distilled water preservation in between. After each demineralization bath, samples of Group A were brushed with Zn-CHA containing toothpaste while samples of Group B were brushed with 1,400 ppm fluoride dentifrice for 5 min before immersion into water. Group C of untreated samples served as control. All the samples were then prepared for scanning electron microscopy (SEM) analysis. A score rating system was used to perform a non-parametric statistical analysis. No statistically significant differences were found between the samples brushed with fluoride toothpaste and those untreated (Groups B and C) where the highest grade of damage was found, while the lowest grade was recorded in the samples brushed with Zn-CHA (Group A) and there was a statistically significant difference between this group and the other two groups.

The study aimed to investigate the morphology and composition of the interproximal reduced enamel after exposition to saliva and casein phosphopeptide amorphous calcium phosphate with sodium fluoride (CPP-ACPF). Fourteen patients undergoing an orthodontic treatment with 4 premolars extractions participated to the study. Interproximal enamel reduction (IER) was performed on mesial surfaces of 3 extractive premolars for each patient while 1 served as untreated control. Premolars were assigned to 4 groups: No-S group, sound enamel as control; S-Ex group, stripped and immediately extracted enamel; S-Sal group, stripped and exposed to saliva enamel; S-CPP group, stripped enamel treated with CPP-ACPF. Teeth were extracted at different times, depending on the group they were assigned to and sliced into mesial and distal halves. Mesial surfaces were subjected to environmental scanning electron microscopy with energy dispersive X-ray spectrometry (ESEM/EDX) and to scanning electron microscopy (SEM) analysis. ESEM/EDX investigations showed no statistically significant differences in the content of calcium and phosphate between the 4 groups. SEM observations showed no difference in the morphological appearance of stripped enamel after 30 days of exposure to saliva and CPP-ACPF. Saliva and CPP-ACPF effects on stripped enamel in vivo showed no difference after 30 days.

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