The wide thermal tolerance range of a eurythermic fish (goldfish, Carassius auratus) was used to evaluate how temperature performance curves derived from maximum heart rate (fH) related to those for aerobic scope. For acclimation temperatures of 12°, 20°, and 28°C, optimum temperatures derived from aerobic scope curves (Topt) were 19.9° ± 0.4°, 19.3° ± 0.8°, and 28.7° ± 0.8°C, respectively. The Arrhenius breakpoint temperatures (TAB) for maximum fH were 21.5° ± 0.6°, 23.8° ± 0.9°, and 24.6° ± 0.5°C, respectively. The TQB (temperature where the incremental Q10 of maximum fH decreased abruptly below 1.9) was 24.0° ± 0.7° and 29.8° ± 0.6°C for the 12° and 28°C acclimation temperatures, respectively, and was within the Topt window (11.5°-30.3° and 26.9°-30.5°C, respectively), but TQB for the 20°C acclimation temperature (27.3° ± 0.6°C) was higher than the Topt window (15.4°-23.2°C). Warm acclimation increased the upper critical temperature (Tcrit; from 37.2° ± 0.7° to 44.7° ± 11.8°C) as well as the temperature that triggered a cardiac arrhythmia (Tarr; from 31.1° ± 0.7° to 39.3° ± 0.4°C). In conclusion, we propose that maximum fH and its associated rate transition temperatures (TAB, TQB, and Tarr) can be used to estimate the upper thermal tolerance of eurythermic as well as stenothermic fish independent of acclimation temperature. All the same, great care is needed with such evaluations. For the goldfish, while TAB and TQB were always within the Topt window for 90% of maximum aerobic scope and Topt was closely associated with TAB for 12°C-acclimated fish, TQB had the closest association after 28°C acclimation, and both TAB and TQB were above the Topt window after 20°C acclimation.
Objective
Campylobacter concisus is a Gram‐negative rod closely related to Helicobacter pylori. We sought to identify gastric biopsies positive for C. concisus that had been misdiagnosed as H. pylori gastritis in our routine surgical pathology practice.
Materials and Methods
We performed a retrospective review of gastric biopsies in our regional microbiology and pathology electronic records to identify cases that were submitted for H. pylori testing in which C. consicus was identified on culture and how many had concurrent biopsies sent to pathology for histologic assessment over a two‐year period (2017–2018). Pathologic findings in the gastric biopsies were reviewed and immunohistochemical staining for H. pylori was performed.
Results
50 of 2191 gastric biopsy specimens submitted to microbiology in 2017–18 grew C. concisus (2.3%), compared to 168 in which H. pylori was identified (7.7%). Twenty‐eight cases had concurrent histology. A total of four cases (three from 2017 and one from 2018) demonstrated organisms morphologically identical to H. pylori in the H&E sections, of which all were H. pylori immunoreactive.
Conclusions
Our case series is the first to demonstrate that C. concisus can mimic H. pylori gastritis in routine biopsy pathology.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.