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A B S T R A C TLatent fingerprint has been commonly used by Brazilian Army forensic experts as an effective tool for personal identification in crime scene investigations. In cases whereas samples are considered unusable for dactyloscopy, genetic analysis appears as a complementary method that prevents waste of expert testimony. The aim of our study was to standardize DNA collection and extraction techniques in two types of surfaces: glass and metal, in order to adapt these methodologies to military reality. Fingerprints were collected using two swabbing solutions: NaCl 0.9% and sodium dodecyl sulfate 2%. DNA was isolated by testing two methods: Lysis solution: SDS/proteinase and QIAmp 1 DNA Investigator 1 Kit (Qiagen). PCR amplification was performed with AmpFLSTR 1 MiniFiler TM Kit (Life Technologies). It was observed that Lysis extraction with NaCl 0.9% swabbing solution gave satisfactory results for samples collected both on glass and metallic supports.

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Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.

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