Description of two novel species, Sphingomonas abaci sp. nov. and Sphingomonas panni sp. nov. Two Gram-negative, rod-shaped, non-spore-forming bacterial strains designated C42 T and C52 T were isolated in the Medical Clinic for Small Animals and Ungulates at the University for Veterinary Medicine Vienna, Austria. On the basis of 16S rRNA gene sequence similarity, both strains were shown to belong to the genus Sphingomonas. Strain C42 T showed the greatest levels of sequence similarity with Sphingomonas melonis DSM 14444 T and Sphingomonas aquatilis KCTC 2881 T (both 97?7 %). Strain C52 T showed the greatest levels of sequence similarity with Sphingomonas koreensis KCTC 2882 T (97?2 %), Sphingomonas aquatilis KCTC 2881 T (97?1 %) and S. melonis DSM 14444 T (97?0 %). The presence of Q-10 as the main ubiquinone, the predominance of the compound sym-homospermidine in the polyamine patterns, the presence of a Sphingomonadaceae-specific sphingoglycolipid in the polar lipid patterns, the presence of the fatty acid 2-OH C 14 : 0 and the lack of 3-hydroxy fatty acids supported the identification of the two novel strains as members of the genus Sphingomonas sensu stricto. Unique physiological characteristics, protein patterns, quantitative differences in their fatty acid profiles and the results of genomic fingerprinting and DNA-DNA hybridizations differentiated strains C42 T and C52 T from closely related Sphingomonas species. Hence, the two strains are described as novel species of the genus Sphingomonas sensu stricto. The names Sphingomonas abaci sp. nov. (type strain C42 T =LMG 21978 T =DSM 15867 T ) and Sphingomonas panni sp. nov. (type strain C52 T =LMG 21979 T =DSM 15761 T ) are proposed.During investigations designed to evaluate hygiene at the Medical Clinic for Small Animals and Ungulates at the University for Veterinary Medicine Vienna, Austria, numerous micro-organisms were isolated. In order to identify significant groups, isolates showing obvious similarities in colony morphology and pigmentation were subjected to comparison of their protein patterns after SDS-PAGE (Altenburger et al., 1996). From protein-similarity groups consisting of at least three strains, a representative was characterized in more detail. On the basis of a preliminary classification, selected representatives of these protein-similarity groups were identified as members of the yeast genus Rhodotorula, the bacterial genera Pseudomonas (Hauser et al., 2004), Acinetobacter, Staphylococcus, Bacillus and Sphingomonas, and coliforms. Here we report on the taxonomic characterization of the orange-pigmented strain C42 T and the yellow-pigmented strain C52 T preliminarily identified as members of the genus Sphingomonas.Strain C42 T was isolated from a treatment table after inoculation on PYE agar (l 21 : 3?0 g peptone from casein, 3?0 g yeast extract, 15?0 g agar; pH 7?2). Strain C52 T was isolated from a wipe in the treatment room after cultivation on PYE agar. Single colonies were visible after cultivation at 28 uC for 48 h.The 16S rRNA gene was...

Three yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterial strains, C36 T , C37 and C39, were isolated in the Medical Clinic for Small Animals and Ungulates at the University for Veterinary Medicine in Vienna, Austria. On the basis of 16S rRNA gene sequence similarity, strain C36T was shown to belong to the genus Pseudomonas; Pseudomonas oleovorans DSM 1045 T was the nearest relative (99?5 % sequence similarity). Other Pseudomonas species shared <97 % sequence similarity with strain C36 T . The presence of Q-9 as the major ubiquinone, the predominance of putrescine and spermidine in its polyamine patterns and its fatty acid profile [i.e. the predominance of C 16 : 0 , summed feature 3 (C 16 : 1 v7c and/or 2-OH C 15 : 0 iso), C 18 : 1 v7c and the presence of 3-OH C 10 : 0 , 3-OH C 12 : 0 and 2-OH C 12 : 0 ] were in agreement with identification of this strain as a member of the genus Pseudomonas. Physiological and biochemical characteristics and the results of genomic fingerprinting clearly differentiated strain C36 T from its phylogenetic relative P. oleovorans DSM 1045 T . Results from DNA-DNA hybridization showed that strain C36 T represents a species that is distinct from P. oleovorans DSM 1045T . These data demonstrate that strain C36 T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas psychrotolerans sp. nov. is proposed. The type strain is C36 T (=LMG 21977 T =DSM 15758 T ). Additionally, physiological, biochemical, chemotaxonomic and genomic fingerprints indicate that P. oleovorans ATCC 29347 may not be a member of the species P. oleovorans sensu stricto.During hygiene investigations at the Medical Clinic for Small Animals and Ungulates at the University for Veterinary Medicine in Vienna, Austria, numerous microorganisms were isolated. To identify significant groups, isolates that showed obvious similarities in colony morphology and pigmentation were compared by analysis of their protein patterns using SDS-PAGE (Altenburger et al., 1996). Protein similarity groups that consisted of at least three strains were characterized in more detail. Based on their preliminary classification, selected representatives of these protein similarity groups were identified as members of the yeast genus Rhodotorula, the bacterial genera Acinetobacter, Staphylococcus, Bacillus, Sphingomonas and Pseudomonas, and coliforms.Taxonomic characterization of three yellow-pigmented strains (C36 T , C37 and C39), of which the representative strain, C36T , was preliminarily identified as a member of the genus Pseudomonas, is reported. Strains C36T and C37 were isolated from water under a dog's cage and strain C39 was isolated from a strip of metal under the treatment table after cultivation on PYE agar (pH 7?2), which contained (l 21 ): 3?0 g peptone from casein, 3?0 g yeast extract and 15?0 g agar. The isolates were subcultivated on PYE agar at 28 u C for 48 h. Pseudomonas oleovorans DSM 1045 T and P. oleovorans ATCC 29347 were kindly provided by G.