Description of two novel species, Sphingomonas abaci sp. nov. and Sphingomonas panni sp. nov.

Busse, Hans‐Jürgen; Hauser, Elke; Kämpfer, Peter

doi:10.1099/ijs.0.63872-0

Cited by 60 publications

(51 citation statements)

References 19 publications

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“…Since the fatty acid profile of strain S8-3 T did not contain other branched fatty acids, which is in agreement with all species of the family Sphingomonadaceae, it is most likely that summed feature 3 is representing exclusively C 16 : 1 v7c and not iso-C 15 : 0 2-OH. The fatty acid profile of strain S8-3 T was in agreement with those of other members of the genus Sphingomonas (Busse et al, , 2003(Busse et al, , 2005Denner et al, 1999;Kämpfer et al, 2007;Ohta et al, 2004;Zhang et al, 2011).…”

supporting

confidence: 79%

Sphingomonas alpina sp. nov., a psychrophilic bacterium isolated from alpine soil

Margesin

Zhang

Busse

2012

International Journal of Systematic and Evolutionary Microbiology

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An aerobic, Gram-negative-staining, motile, psychrophilic bacterium, designated strain S8-3 T , was isolated from alpine soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S8-3 T was related to the genus Sphingomonas and had highest 16S rRNA gene sequence similarity with Sphingomonas oligophenolica S213 T (98.0 %). 16S RNA gene sequence similarity between strain S8-3 T and Sphingomonas paucimobilis ATCC 29837 T (the type species of the genus Sphingomonas) was 93.0 %. Strain S8-3 T contained Q-10 as the ubiquinone and C 18 : 1 v7c (65.0 %) and C 14 : 0 2-OH (13.4 %) as the dominant fatty acids (.10 %). The major polyamines were the triamine sym-homospermidine and spermidine. The polar lipid profile contained sphingoglycolipid, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The DNA G+C content was 64.1 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain S8-3 T is a representative of a novel species of the genus Sphingomonas, for which the name Sphingomonas alpina sp. nov. is proposed. The type strain is S8-3 T (5DSM 22537 T 5LMG 26055 T ).The genus Sphingomonas was first proposed by Yabuuchi et al. (1990) and has been emended subsequently by Takeuchi et al. (1993Takeuchi et al. ( , 2001, Yabuuchi et al. (2002) and Busse et al. (2003) to accommodate aerobic, Gramnegative and non-spore-forming bacteria that are characterized chemotaxonomically by the absence of 3-hydroxy fatty acids and by the presence of 2-hydroxymyristic acid (C 14 : 0 2-OH), Q-10 as the respiratory quinone and symhomospermidine as the key polyamine. So far, the nomenclature of Takeuchi et al. (2001) is generally used (Busse et al., 2003). Strain S8-3 T was isolated from alpine soil collected in the Hohe Tauern/Grossglockner area (Piffalpe) on the north slope at 1500 m above sea level . The strain was isolated as previously described (Zhang et al., 2010). One of the pure cultures was yellow-pigmented and was designated S8-3 T . Strain S8-3 T was routinely cultured on R2A agar (containing 0.05 % yeast extract, 0.05 % peptone, 0.05 % Casamino acids, 0.05 % glucose, 0.05 % starch, 0.03 % sodium pyruvate, 0.03 % K 2 HPO 4 , 0.005 % MgSO 4 , 1.5 % agar; pH 7; Reasoner & Geldreich, 1985) at 20 u C, and stored as a suspension in skimmed milk (10 %, w/v) at 280 u C. Sphingomonas echinoides DSM 1805 T , Sphingomonas oligophenolica S213 T and Sphingomonas glacialis C16y T (5DSM 22294 T ; Zhang et al., 2011) were routinely grown on R2A agar at 20 u C and used as reference strains.DNA of strain S8-3 T was extracted and purified as described by Sambrook et al. (1989). The 16S rRNA gene was amplified by PCR (Margesin et al., 2012) with a pair of universal primers, 27F (59-AGAGTTTGATCCTGGCTC-AG-39) and 1541R (59-AAGGAGGTGATCCAGCCGCA-39). The amplification product was cloned into vector pGEM-T Easy (Promega), and the recombinant plasmid was propagated in Escherichia coli according to the manufacturer's instructions ...

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supporting

confidence: 79%

Sphingomonas alpina sp. nov., a psychrophilic bacterium isolated from alpine soil

Margesin

Zhang

Busse

2012

International Journal of Systematic and Evolutionary Microbiology

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“…The bootstrap values were set as 1000 replications and evolutionary distances were calculated using Kimura's two-parameter model (Kimura, 1980). Growth of strain 6NM-7 T was observed on different media, including nutrient agar (NA; Huankai), tryptic soy agar (TSA; Huankai), R2A agar (Qingdao Hope) and PYE agar (Busse et al, 2005), at 30 8C for 7 days. Colony and cell morphology were observed by eye and with a transmission electron microscope (H7650; Hitachi), respectively, after 3 days on R2A agar at 30 8C.…”

mentioning

confidence: 99%

Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing

Feng

Yang

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A heavy metal-resistant and dimethyl disulfide-producing bacterial strain, designated 6NM-7 T , was isolated from wolfram mine tailing, Dayu County, Jiangxi Province, PR China. Strain 6NM-7 T was aerobic, Gram-stain-negative and motile by means of a single polar flagellum. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain 6NM-7 T was affiliated with the genus Massilia and was closely related to Massilia norwichensis LMG 28164 T (98.8 % 16S rRNA gene sequence similarity), Massilia kyonggiensis KACC 17471 T (98.4 %), Massilia niastensis KACC 12599 T (97.8 %), Massilia tieshanensis KACC 14940 T (97.3 %), Massilia haematophila KACC 13771 T (97.2 %), Massilia namucuonensis CGMCC 1.11014 T (97.1 %) and Massilia aerilata KACC 12505 T (97.1 %). The DNA-DNA relatedness values between strain 6NM-7 T and its closely related type strains were all below 70 %. The major respiratory quinone was unbiquinone 8 (Q-8) and the major cellular fatty acids consisted of C 16 : 0 (33.2 %), summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH; 21.8 %), C 17 : 0 cyclo (20.8 %), C 18 : 1 v7c (7.4 %) and C 10 : 0 3-OH (5.8 %). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of strain 6NM-7 T was 66.8¡0.6 mol%. On the basis of the results of this polyphasic taxonomic study, strain 6NM-7 T should be assigned to a novel species of the genus Massilia, for which the name Massilia putida sp. nov. is proposed. The type strain is 6NM-7 T (5DSM 27523 T 5KCTC 42761 T ).The genus Massilia was first described by La Scola et al. (1998) and more than 25 species have been reported (http://www.bacterio.net/massilia.html), including the recently described species, Massilia arvi (Singh et al., 2015), Massilia eurypsychrophila (Shen et al., 2015) and Massilia norwichensis (Orthová et al., 2015). Members of the genus Massilia are characterized by Gram-negative, aerobic, non-spore-forming, motile rods with Q-8 as major reparatory quinone. In this paper, we reported a novel species of the genus Massilia, which can produce dimethyl disulfide (DMDS). As methyl bromide (CH 3 Br) is a potent ozone depletor and scheduled to be phased out by all countries in 2015 (Dugravot et al., 2003), DMDS has been recommended as an alternative fumigant to control high infestation levels of soil-borne pathogens, nematodes and weeds (Coosemans, 2005;Fritsch, 2005;Heller et al., 2009). In addition, DMDS-producing microbes have the potential to be used for controlling plant foliar diseases and promoting plant growth by enhancing the availability of reduced sulfur (Huang et al., 2012; Meldau et al., 2013). To our knowledge, rarely have novel species been reported to produce DMDS. Strain 6NM-7 T was isolated from a wolfram mine tailing sample from Dayu County (1148 209 060 E 258 279 370 N), Jiangxi Province, PR China. The total Cu, Pb, Zn and Cd concentrations of the tailing sample were 198.9, 149.4, 340.4, and 9.3 mg kg 21 , respectively, and the pH was 6.95. Isolat...

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“…The G+C content of most of the other species of the genus Jeotgalicoccus was iso-C 13 : 0 0.6 (1.9) 0.1 iso-C 14 : 0 1.5 1.1 (1. For polyamine, quinone and polar lipid analyses cells were grown in PYE medium (Busse et al, 2005) at 28 C. For analysis of polyamines (Altenburger et al, 1996;Busse et al, 1997) biomass was harvested at the lateexponential growth phase. Quinone and polar lipid extraction and analyses were carried out as described by Tindall (1990a, b) and Altenburger et al (1996).…”

mentioning

confidence: 99%

Jeotgalicoccus schoeneichii sp. nov. isolated from exhaust air of a pig barn

Glaeser

Kleinhagauer

Jäckel³

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A Gram-staining-positive, non-motile, non-spore-forming, coccus (strain 140805-STR-02 T ) was isolated from exhaust air of a pig barn on Columbia Blood Agar Base (Oxoid) supplemented with 5 % defibrinated horse blood, Streptococcus selective supplement and 0.5 mg erythromycin l À1 . The strains shared high 16S rRNA gene sequence similarity to Jeotgalicoccus pinnipedialis (98.6 %) but only a maximum of 94 % sequence similarity to all other species of the genus Jeotgalicoccus. DNA-DNA hybridisation values between strain 140805-STR-02 T and J. pinnipedialis CIP 107946 T were 60.3 % (reciprocal, 51.2 %). The quinone system of 140805-STR-02 T contained predominantly menaquinone MK-7 and minor amounts of MK-6. The polar lipid profile of strain 140805-STR-02 T contained the major compounds diphosphatidylglycerol and phosphatidylglycerol and four unidentified lipids present in minor to moderate amounts. In the polyamine pattern spermidine and spermine were predominant. The fatty acid profile comprising iso-C 15 : 0 and anteiso-C 15 : 0 as major fatty acids, and was in congruence with those reported for other species of the genus Jeotgalicoccus and thus supported the affiliation of strain 140805-STR-02 T to this genus. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain 140805-STR-02 T from the most closely related species. Strain 140805-STR-02 T represents a novel species, for which the names Jeotgalicoccus schoeneichii sp. nov. is proposed, with the type strain 140805-STR-02 T (=LMG 29445 T =CCM 8667 T ). In this polyphasic taxonomic study, strain 140805-STR-02 T isolated from exhaust air of a pig barn was investigated, which is phylogenetically very closely related to J. pinnipedialis. The genus

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Description of two novel species, Sphingomonas abaci sp. nov. and Sphingomonas panni sp. nov.

Cited by 60 publications

References 19 publications

Sphingomonas alpina sp. nov., a psychrophilic bacterium isolated from alpine soil

Sphingomonas alpina sp. nov., a psychrophilic bacterium isolated from alpine soil

Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing

Jeotgalicoccus schoeneichii sp. nov. isolated from exhaust air of a pig barn

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