Optimizing DNA polymerases for a broad range of tasks requires an understanding of the factors influencing polymerase fidelity, but many details of polymerase behavior remain unknown, especially in the presence of mismatched nascent base pairs. Using molecular dynamics, the large fragment of Bacillus stearothermophilus DNA polymerase I is simulated in the presence of all 16 possible standard nucleoside triphosphate-template (dNTP-dN) pairs, including four Watson-Crick pairs and 12 mismatches. The precatalytic steps of nucleotide addition from nucleotide insertion to immediately preceding catalysis are explored using three starting structures representing different stages of nucleotide addition. From these simulations, interactions between dNTPs and the DNA-protein complex formed by the polymerase are elucidated. Patterns of large-scale conformational shifts, classification of nucleotide pairs based on composition, and investigation of the roles of residues interacting with dNTPs are completed on 50+ μs of simulation. The role of molecular dynamics in studies of polymerase behavior is discussed.

Key informant interviews with extension-supported community coalition members in five rural communities during the COVID-19 pandemic were used to examine the unique characteristics of rural community resiliency in the face of a crisis based on the community capitals framework. Using a thematic analysis, seven different human and material capitals were examined in community members’ reactions to a “black swan” event. Rural community coalition members shared their perspectives on the vitality of their community in the face of adversity which revealed obstacles extension professionals can support through community development efforts focused on building resiliency. The analysis identified three emergent themes: (1) juxtaposing restrictions and uncertainties with unexpected successes; (2) demonstrating resiliency through connectedness; and (3) correlating community health with changing perceptions of COVID-19. Additionally, each theme was juxtaposed with the interplay of community capitals as they related to coalition efforts in a pandemic that can assist if further developing health communication and extension education efforts within rural communities across the globe in times of crisis. Keywords: rural; resiliency; Community Capitals Framework; health communication; COVID-19

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