To evaluate the effects of treatment with Indigo Carmine (IC) on rat livers subjected to ischemia-reperfusion injury. Methods: The animals were subdivided into 4 groups: 1.SHAM group(SH)-saline; 2.SHAM group with IC-2mg/Kg(SHIC); 3.IR group-rats submitted to ischemia and reperfusion with saline(IR); 4.IR group with IC-2mg/Kg(IRIC). The IR protocol consists of liver exposure and administration of drug or saline intravenously, followed by 60 minutes of ischemia and 15 of reperfusion. Liver samples were collected for biochemical analysis. Results: State 3 of mitochondrial respiration showed a significant worsening of the IRIC group in relation to all others. State 4 showed a difference between IRIC and SHIC. The Respiratory Control Ratio showed statistical decrease in IR and IRIC versus Sham. The osmotic swelling showed significant difference between SHxIR; SHICxIRIC and SHxIRIC. There was a significant increase in ALT in the IRIC group in relation to all the others. Concerning the nitrate dosage, there was a decrease in the group treated with IC(IRxIRIC). There was no difference regarding the dosage of Malondialdehyde. Conclusion: IC was not able to protect mitochondria from IR injury and proved to be a potentiating agent, acting in synergy with the IR injury promoting damage to the hepatocyte membranes.

Background: The COVID-19 pandemic and its control measures, which have not been experienced in the world in the last hundred years, have impacted academic scientific production, which, in Brazil, was already in the process of progressive erosion of its foundations. Thus, scientific initiation during medical graduation, which depends on funding and institutional structure, and extremely beneficial to the graduation process, was jeopardized due to restrictive measures. Objective: This article aims to expose the point of view of undergraduate medical students enrolled in a scientific initiation program about the panorama of Brazilian academic scientific production and the impact of the COVID-19 pandemic in its multiple aspects. Discussion: The lack of an effective scientific initiation is one of the many factors that lead to a decline in the number of medical researchers. The way that scientific initiation is placed, as part of a parallel curriculum, and a whole scrapped public production structure are impairing, chronic and noteworthy features. The apparatus of national scientific production is mostly structured in Higher Education Institutions and research institutions, both public. Within the scope of graduation, it can be didactically subdivided into four pillars: financial support, structure, student proactivity and the advisor's aptitude. The COVID-19 pandemic has been an additional blow to this weakened and fragile structure. Scientific initiation was thus negatively impacted. Public opinion and political aspects further influence an imbroglio of “scientific denial” and a craving for effective information and solutions to the unprecedented problem of a pandemic of this proportion. Conclusion: It is clear that national scientific production is placed in a survival situation in the face of new challenges posed by the pandemic. Likewise, scientific initiation is less and less stimulating during graduation, even though it is an experience of great value in medical and personal development.