The probiotics are being used as ecofriendly and bioremediation tools for developing sustainability to aquaculture. The present study was conducted to explore the practical capability of using dietary lactic acid bacteria (Pediococcus acidilactici) probiotics and see how its dose variation affected the water quality, growth performance, survival rate, body composition, blood biochemical parameters, and intestinal histology of European sea bass (Dicentrarchus labrax L.). A total of 120 fingerlings with an initial weight of 9 ± 0.2  g were divided into four groups, each with three replicates. The feeding experiment lasted for 60 days. In addition to the control (without probiotics) (T0), fish were fed diets containing (T1) 2.0, (T2) 2.5, and (T3) 3.0 g of probiotics per kg of diet twice a day. When compared to the control, sea bass fed probiotic-supplemented diets had significantly higher growth parameters, fish body “crude lipid,” and villi height ( p < 0.05 , p < 0.01 , and p < 0.001 ). The P. acidilactici probiotic treatments improved survival rate, feed conversion ratio, body composition, and blood biochemical markers, but not statistically significant ( p > 0.05 ). Also, in regard to water quality, P. acidilactici drastically reduced ammonia and pH levels. In this experiment, fish fed with a dosage of 3.0 g of this commercial probiotic per kg of probiotics performed better. The study found that including probiotics in the diets of European sea bass improved growth, body composition, survival rate, blood biochemical markers, intestinal histology, and some water quality parameters.