Aim:This study aimed to evaluate the effects of Meniran extract (Phyllanthus niruri L.) administration on leukocyte profile of broiler chickens infected with Mycoplasma gallisepticum.Materials and Methods:Thirty broiler chickens, 21 days old were divided into five treatment groups. P0 (−): Chickens without any treatment; P0 (+), P1, P2, and P3: Chickens were infected with M. gallisepticum 108 cells/ml/animal orally, then given no treatment, Meniran extract 60%, 62.5%, and 65% orally at a dose of 1 ml/kg body weight, respectively. The treatment of Meniran extract was given for 7 days.Results:Leukocyte count with the lowest number showed in Group P0 (−) and Group P3 (p>0.05). Increased number of basophils was found in Group P0 (+), Group P1, and Group P2. The highest number of heterophils was found in Group P0 (+) and was significantly different from Group P0 to P3 (p<0.05). The same pattern was also seen in the number of lymphocytes in all treatment groups. The number of monocytes showed no significant difference between all treatment groups (p>0.05).Discussion:Increased the number of leukocytes is often observed in inflammation due to general infections, trauma, or toxicity. Shifting in the number of heterophile or lymphocytes, an increase in the number of monocytes, basophils, and eosinophils may also be associated with various infectious or inflammatory conditions. Heterophils play a role as an antibacterial defense through several effective mechanisms. When infections and inflammation occur, the heterophils will increase to phagocytosis microbe.Conclusion:It can be concluded that Meniran extract (P. niruri L.) at a dose of 65% can decrease the total number of leukocytes in broilers infected with M. gallisepticum.

Alkaloid test: Conducted by the method of Mayer, Wagner and Dragendorff. A sample of 3 mL was added with 5 mL of 2 M HCl, stirred and cooled at room temperature. After that, 0.5 g of NaCl was added to the already cool sample then stirred and filtered. The filtrate obtained was added with drops of 2 M HCl, then separated into 4 parts (A, B, C, D). Filtrate A was used as blank, filtrate B was added to Mayer's reagent, filtrate C was to be added to Wagner reagent, while filtrate D was used for the confirmation test. Precipitate would be formed on the addition of Mayer and Wagner reagents, when identification indicates the presence of alkaloid. Confirmation test was carried out by adding 25% ammonia to filtrate D until PH changed to 8-9. Then chloroform was added, and evaporated in water bath. Then 2M HCl was added, stirred and filtered. The filtrate was divided into 3 parts. Filtrate A was used as blank, filtrate B was tested with Mayer's reagent, while filtrate C was tested with Dragendorff's reagent. The formation of a precipitate indicated the presence of alkaloid.Tannin test: 2 grams of sample was put into a mL boiling flask, then added 350 mL of distilled water and refluxed for 3 hours. The sample was cooled and transferred into 500 ml volumetric flask, then filtered and 2 ml of the filtrate was put into a 100 ml volumetric flask. 2 ml of Folin Denis reagent and 5 ml of saturated Na 2 CO 3 were added and allowed to stand for 40 minutes then the absorbance was measured at wavelength of 725 nm.

No abstract

No abstract

No abstract