: Micropropagation and callus culture of Daucus capillifolius Gilli (Apiaceae) were successfully established in Murashige and Skoog(MS) solid medium enriched with different plant growth regulators. The combination of NAA (1mg/L) + BAP (0.1mg/L) was found to be the optimum for both organogenesis and embryogenesis. The compositions of essential oils obtained from wild fruits, cultivated fruits and callus culture were analyzed by GC-MS. The major identified constituents of the essential oils of wild and cultivated fruits were geranyl acetate (31.84%, 32.49%), α-azarone (21.69%), β-azarone (20.63 %), trans methyl isoeugenol (11.0%, 7.48%), α-humulene (7.25%, 4.35%), juniper camphor (4.71%, 4.26%) and α-pinene (3.26%, 3.63%), respectively. When screened, the essential oil of the wild fruits exhibited potent cytotoxic effect against Hep-G2 with IC5014.9 µg/mL and moderate activity against MCF-7 and HCT-116 cells with IC50 (29.1and 59.8 µg/mL), respectively. Meanwhile, the essential oil of the cultivated fruitsexhibited moderate activity against Hep-G2, 53.7 and 48.0 µg/mL), respectively.