No abstract

Background: Cancer is abnormal cells growth that is responsible for the majority of global deaths and cancer is expected to rank as leading cause of death and the single most important barrier to increasing life expectancy in every country of the world in the 21st century. According to the estimates from the world health organization in 2015, cancer is the first or second leading cause of death before age 70 years in 91 of 172 countries, and it ranks third or fourth in an additional 22 countries. Many anti-cancer drugs in current use such as paclitaxel, vincristine, and vinblastine are naturally derived agents. The aim of this study is to determine the effects of ethanolic leaves extract of Moringa oleifera and docetaxel each separately and in combinations on the proliferation of LNCaP cell line, and on the level of tumor necrosis factor related apoptosis induced ligand (TRAIL). Methods: This work was performed in the laboratory of cancer research at college of medicine\ university of Babylon during the period from January 2021 to October 2021. The study was approved by the ethics committee in the college of medicine at university of Babylon.

Silver nanoparticles (AgNPs) are of special concern as a result of their unique chemical, physical and biological characteristics. It has become an attractive alternative to antibiotics due to their broad-spectrum antimicrobial activity.The study aimed to determine the antibacterial activity of AgNPs against S. aureus bacteria and the effect of AgNPs on the viability of normal cell line (vero cell). A total of 70 clinical samples (wound and vagina swab, stool and urine) were used in this study. Bacterial isolates were subjected to the microscopical, cultural and biochemical evaluation.AgNPs were prepared and checked for their antimicrobial activity by the use of various concentrations employing agar dilution method. In addition, the effect of different concentrations of AgNps on a viability of vero cells was examined. The results showed that out of 70 clinical samples, 11 (15.7%) isolates were Staphylococcus aureus.AgNps showed high activity against S. aureus at concentrations (100 μg/ml and 200μg/ml). It was found that there was no effect of AgNPs on the viability of the normal vero cells at (≤ 250 μg/ml) concentration, but they have cytotoxic effect on the viability of the these cells at high concentrations. This study concluded that AgNPs possess good antimicrobial activity and the concentrations that maintain the cell viability could be used as an alternative therapy to treat S. aureus infections.