Highlights d Cancer-associated fibroblasts contribute to pancreatic cancer heterogeneity d Cancer cells can have a double-positive phenotype: proliferation and invasion d High CAF abundance linked with DP cells enriched for MAPK and STAT3 co-signaling d Intra-tumoral gland types provide tissue heterogeneity linked with clinical outcome

Graphical Abstract Highlights d Extracellular vesicles (EVs) shed by glioma cells are taken up by microglia in vivo d miR-21 is functionally transferred from glioma to microglia through EVs in vivo d miR-21 mRNA targets in microglia are downregulated upon EV uptake d Microglia proliferation is increased after miR-21 target Btg2 downregulation

Highlights d Mono-phosphorylation controls RB's association with other proteins d Distinct mono-phosphorylated forms of RB have different transcriptional outputs d RB mono-phosphorylation at S811 promotes NuRDdependent transcriptional repression d Mono-phosphorylation of RB's C-terminal domain enhances expression of OXPHOS genes