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The inactivation of homozygous yeast strains of different ploidy by thiopyronine in presence and in absence of visible light and the effeciency of this dye upon nucleic acids and nucleic acid components was investigated. Some experiments of the mutation activity of thiopyronine on Sac charomyces are described. The inactivation of yeast in presence of visible light is not dependent on ploidy; in absence of visible light there is a remarkable increase of inactivation with increasing of ploidy.