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Callus induction of the agarophyte marine alga Gracilaria verrucosa was obtained on modified ASP6 F2 media. The formation of callus from axenic explants depended on the addition of glycerol (0.217 mol 1 -1) and plant growth regulators (2.0 mg 1 ' IPA, 2.0 mg 1 -1 IAA, and 1.0 mg 1-1 2iP). Glycerol alone, did not induce callus. The addition of 5.0 mg 1 DPU (Diphenylurea) caused callus induction on the whole surface of the explants of Gracilaria verrucosa. Different culture-media, carbon sources and phytohormones were tested. The application of floridoside (2-O-D-glycerol-alpha-D-galactopyranoside), the main product of photosynthesis of the red algae, did not promote growth and callus induction.

MEGNET, RIOLAND (Institut fuir Allgemeine Mikrobiologie der Uiniversitat, Bern, Switzerland). Effect of 2-deoxyglucose on Schizosaccharomyces pombe. J. Bacteriol. 90:1032-1035. 1965.-Cultivation of Schizosaccaromyces pombe in a medium containing 2-deoxyglucose (100 MAg/ml) results in the death of the cells after an initial period of apparently normal growth. At higher deoxyglucose concentration (400 ,g/ml), the cells die immediately after inioculation. Only growing cells are killed, and microscopic inspection of the cultures reveals cell-wall fragments of lysed cells. A mutant resistant to 2-deoxyglucose, which cannot use glucose as a carbon source, was found to be partially deficient in hexokinase. The data constitute evidence for the inhibition of some reaction(s) in the synthesis of cell-wall polysaccharides by metabolites of 2-deoxyglucose in this organism.

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