Effect of 2-Deoxyglucose on
            <i>Schizosaccharomyces pombe</i>

Megnet, Roland

doi:10.1128/jb.90.4.1032-1035.1965

Cited by 73 publications

(31 citation statements)

References 6 publications

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“…The loss of the normal cellular configuration, and the granular appearance of C. paramecium after being inoculated into 2-DG media suggests a hypothesis formulated to explain some results in yeasts in which cell walls became fragile, resulting in lysis and death due to inhibition of the synthesis of capsular polysaccharides. The trapping of uridine nucleotides used in the synthesis of polysaccharides by the phosphorylated derivatives of 2-deoxysugars is the reaction believed to account for this effect (8,14).…”

Section: Discussionmentioning

confidence: 99%

Effect of Hexose Analogs on Growth of Chilomonas paramecium

Ukeles

1970

The Journal of Protozoology

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SYNOPSIS. Observations on the effect of 7 glucose analogs on growth of C. paramecium showed that 2‐deoxy‐D‐glucose and 2‐deoxy‐D‐galactose inhibited growth. Inhibition in 2‐DG was not influenced by pH, calcium concentration, or by carbon sources as lactate, acetate, n‐butyl alcohol, or succinate. Similarly, glucose or glucose‐6‐phosphate did not reverse inhibition. Organisms were viable in 2‐DG only when a carbon source in the basal medium was absent. Wherever a useful carbon source was present, 2‐DG was lethal in concentrations over 40 mg/100 ml. Phosphorylation of 2‐DG did not occur. Lactic acid dehydrogenase was not inhibited. Glucose‐6‐phosphate dehydrogenase appears to have an affinity for 2‐DG; phosphohexose isomerase activity was decreased by 2‐DG.

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Section: Discussionmentioning

confidence: 99%

Effect of Hexose Analogs on Growth of Chilomonas paramecium

Ukeles

1970

The Journal of Protozoology

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“…Mutants were isolated using 2 methods. In method 1, which depended upon 2-deoxyglucoseinduced death of growing cells [11,12], 0.1-ml samples of mutagen-treated cell suspension were spread onto plates of EMM2(I% GLY;DOG). After incubation for 10 days at 30 o C, the edge of the agar medium of each plate was lifted at one point and sufficient sterile glucose solution was run under the agar gel to give a concentration of approximately 1% after the glucose had diffused into the medium.…”

Section: Mutagenesis and Isolation Of Mutantsmentioning

confidence: 99%

Mutants of Schizosaccharomyces pombe defective in glycerol catabolism

Kong

1987

FEMS Microbiology Letters

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“…It is well established that deGlc adversely affects cell wall synthesis in yeast. This is undoubtedly the basis of the fungicidal action that this compound has on yeasts, since sensitivity was proportional to the mean rate of extension of the individual yeast cells (Johnson and Rupert, 1967); only growing cells were killed and fragments of cell wall were found in the treated suspensions (Megnet, 1965). Lysis occurred at points coinciding with the regions of growth of the glucan layers of the yeast cell wall (Johnson, 1968a).…”

Section: Mechanisms Of Inhibition : 2-deoxy-d-glucose and Related Sugarsmentioning

confidence: 99%

Effects of Hexose Analogues on Fungi: Mechanisms of Ingibition and of Resistance

Moore

1981

New Phytologist

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I. SUMMARY Although the effects of a number of sugar analogues are discussed in this review, most attention is devoted to 2‐deoxy‐D‐glucose (deGlc) and L‐sorbose. The growth of fungi is not uniformly inhibited by sugar analogues since some species or strains can metabolize sugars which severely inhibit others. Sugar analogues usually cause inhibitions by being used in metabolism in place of glucose. Both deGlc and sorbose are metabolized by several species but, in general, only a small fraction of the analogue is utilized. The uptake and phosphorylation of readily metabolized sugars are inhibited by deGlc. Inhibitions by sugar analogues occur on media containing non‐carbohydrate sources of carbon, so inhibition of sugar uptake is unlikely to be a major component of the growth inhibition which occurs on sugar‐containing media. Hexokinase phosphorylates deGlc, and its 6‐phosphate inhibits the activity of especially phosphohexose isomerase and glucose 6‐phosphate dehydrogenase. Apart from an involvement in synthesis of more complex sugars, formation of deGlc‐6P is the usual limit of metabolism of this sugar analogue. Accumulation of the phosphate ester leads to a considerable drain on phosphate pools and ATP levels decline drastically. Indeed, deGlc rapidly initiates degradation of purine nucleotides which can proceed at least to the level of hypoxanthine. In Saccharomyces cerevisiae, deGlc can be condensed to dideoxytrehalose and, in most fungi, polysaccharide synthesis is affected. Preformed wall material is eroded and synthesis of new wall components is prevented by sequestering of uridine and guanosine nucleotides through reaction with deGlc. Interference with the structure of the wall greatly reduces osmotic stability, and cell lysis is the major cause of inhibition of growth by deGlc in yeasts and filamentous fungi alike. L‐Sorbose is not phosphorylated, and the biochemical basis of the inhibitions caused by this analogue is obscure. Some enzymes involved in polysaccharide synthesis are sensitive to inhibition by sorbose, and a characteristic of growth on this sugar is formation of an abnormally thick cell wall. Filamentous fungi grown on solid medium containing sorbose assume an abnormal growth form in which cells are much shortened and branching frequency is increased. There are indications that sorbose, perhaps by interfering with cell wall structure, affects hyphal morphogenesis and that this effect may be magnified into a macroscopic change in the characteristics of the colony during growth on solid medium by secondary environmental effects. Mutants, resistant to deGlc, have alterations in either transport, hexokinase or phosphatase functions, thus emphasizing the important of accumulation of deGlc‐6P in the inhibitions caused by this analogue. Generalization is less easy about mutants resistant to sorbose. The majority are defective in transport, but others show defects in phosphoglucomutase and polysaccharide synthases and there are some correlations with morphological changes which underline the involv...

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Effect of 2-Deoxyglucose on Schizosaccharomyces pombe

Cited by 73 publications

References 6 publications

Effect of Hexose Analogs on Growth of Chilomonas paramecium

Effect of Hexose Analogs on Growth of Chilomonas paramecium

Mutants of Schizosaccharomyces pombe defective in glycerol catabolism

Effects of Hexose Analogues on Fungi: Mechanisms of Ingibition and of Resistance

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