To examine the cytotoxicity of calcium hydroxide on human umbilical cord mesenchymal stem cells (HUCMSC) to understand the characteristics for use in regenerative dentistry procedures especially regenerative endodontics. Material and Methods: HUCMSC was isolated, cultured, and confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSC was cultured and divided into two groups, the control group (cultured in minimum essential medium (MEM) alpha) and calcium hydroxide group (cultured in MEM alpha and calcium hydroxide). Methyl-thiazole-tetrazolium (MTT) assay was done on different concentrations of calcium hydroxide (0.39 to 25 µg/mL) and the cells were observed and counted. One-way ANOVA test was used with a significance level set at 5%. Results: Flow cytometric analysis confirmed positive of CD73, CD90, CD105, negative of CD45 and CD34. A significant difference was found between the concentration of 6.25 and 3.125 µg/mL (p=0.004). There was no significant difference among 6.25, 12.5 and 25 µg/mL concentrations. There was also no significant difference among 0.39, 0.78, 1.56, and 3.125 µg/mL concentrations. Conclusion: Even though calcium hydroxide is a medicament of choice in clinical endodontics, it decreases the viability of HUCMSC. The lower the concentration of calcium hydroxide, the higher the viability of HUCMSC.

How to cite this article: Alida A, Winoto ER, Narmada IB. Receptor activator of nuclear factor-kappa ligand and osteoprotegerin expressions on hyperglycemic Wistar rats (Rattus Novergicus) during orthodontic tooth movement.

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In the absence of the mandibular first molar, there is corresponding supra-eruption of the maxillary first molar, which can lead to occlusal interferences compromising the space available for restoration with a prosthesis. In this case report, the maxillary left first molar intrusion has been discussed with the use of two miniscrews. A 28-year-old female reported with a missing mandibular left first molar and wanting to replace it with a dental implant. The patient presented with a Class I skeletal relation, mild proclination of the anteriors and occlusal contacts. The maxillary first molar had erupted by >4 mm beyond the occlusal plane, thereby reducing the space available for placement of a lower prosthetic crown. After 5 months of intrusion, the desired occlusal clearance was achieved, and impressions were recorded. For retention, the patient was given an upper clear vacuum retainer and the crown was placed on the implant.