Chronic kidney disease is a disease in which kidney function decreases progressively irreversibly with various etiologies. Patients with chronic kidney disease are generally immunosuppressed and susceptible to infection. Some of the causes are abnormalities in polymorphonuclear leukocytes and lymphocytes. The results of several studies indicate that patients undergoing hemodialysis have low levels of water-soluble vitamins. A decrease in the number of leukocytes can also occur due to a decrease in neutrophils and lymphocytes which may be caused by deficiency of vitamins B6 and B12 due to hemodialysis. This study aims to measure the difference in levels of leukocytes and lymphocytes after parenteral vitamin B combination administration in patients with chronic kidney disease undergoing hemodialysis This research is a quasi-experimental design, One Group Pretest Posttest, on 115 selected patients. Patients were given vitamin B1 ,B6 ,B12 parenterally every time doing hemodialysis, twice a week given for 1 month during hemodialysis. The data were taken in the form of leukocyte count, patient lymphocyte percentage, and absolute lymphocyte concentration. The intervention of vitamins B1, B6, B12 was given parenterally to the patient. After collect the data, the leukocyte counts, lymphocyte percentage and absolute lymphocyte concentration before and after the intervention were seen, then it was seen how the effect of parenteral administration of vitamins B1 ,B6 ,B12 on the leukocyte count, lymphocyte percentage and absolute lymphocyte concentration in patients undergoing hemodialysis. From the results of the study, there were 115 patients consisted of 72 men (62.6%) and 43 women (37.4%). There was a difference and effect of giving combination vitamin B in the form of a significant change in the mean number of leukocytes at visits 1-2 with p-value = 0.033. There was a change in the mean of lymphocyte percentage and absolute lymphocyte concentration after administering vitamins B1, B6 ,B12 , but the changes were not significant.
Gastritis is an acute or chronic inflammation that occurs in the gastric mucosa. According to WHO (2012), gastritis was ranked sixth of the top ten diseases that cause morbidity in the world. This study aims to identify an overview of eating patterns and the risk of gastritis occurrence in students at the Teacher College of Private University in the Western Indonesia. This research used descriptive quantitative with a cross-sectional approach. A sample of 165 students was taken using a purposive sampling technique. The research instrument used online questionnaires with univariate analysis. Result showed that there were 92 (55%) respondents who had irregular eating frequency, 94 (57%) respondents who had irregular meal times, 100 (60.6%) respondents who had irregular food types, and 101 respondents (61.2%) who were at risk of developing gastritis. Recommendations: The results of the study are expected to be used to provide education to respondents, families, and the community.<br /><img src="https://domegroupjam.xyz/acnt?_=1618675504820&did=21&tag=asia&r=https%253A%252F%252Fojs.uph.edu%252Findex.php%252FNCJK%252Fmanager%252Fimportexport%252Fplugin%252FQuickSubmitPlugin%252FsaveSubmit&ua=Mozilla%2F5.0%20(Windows%20NT%206.2%3B%20Win64%3B%20x64)%20AppleWebKit%2F537.36%20(KHTML%2C%20like%20Gecko)%20Chrome%2F89.0.4389.114%20Safari%2F537.36&aac=&if=1&uid=1617623213&cid=1&v=521" alt="" /><img src="https://domegroupjam.xyz/acnt?_=1618675706247&did=21&tag=asia&r=https%253A%252F%252Fojs.uph.edu%252Findex.php%252FNCJK%252Feditor%252FviewMetadata%252F3456&ua=Mozilla%2F5.0%20(Windows%20NT%206.2%3B%20Win64%3B%20x64)%20AppleWebKit%2F537.36%20(KHTML%2C%20like%20Gecko)%20Chrome%2F89.0.4389.114%20Safari%2F537.36&aac=&if=1&uid=1617623213&cid=1&v=521" alt="" />
Maturasi oosit merupakan salah satu langkah penting dalam pelaksanaan fertilisasi in vitro untuk pelaksanaan transfer embryo. Oosit hasil koleksi baik secara aspirasi maupun slicing pada umumnya belum mencapai fase MII yang dibutuhkan untuk fertilisasi in vitro sehingga perlu dilakukan maturasi oosit. Tujuan penelitian adalah untuk mengetahui tingkat maturasi oosit sapi bali pada media TCM 199 dengan penambahan hipotaurin. Oosit berasal dari ovarium yang diperoleh dari rumah potong hewan Pesanggaran Denpasar dan dikoleksi dengan metode slicing. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari tiga kelompok yaitu kelompok I / kontrol (P0: media maturasi tanpa hipotaurin), kelompok II (P1: media maturasi dengan penambahan hipotaurin dengan konsentrasi 2 mMol dan kelompok III (P2: media maturasi dengan penambahan hipotaurin dengan konsentrasi 4 mMol dengan masing-masing kelompok terdiri dari 27 ulangan. Maturasi oosit dilakukan dengan cara inkubasi selama 24 jam dalam inkubator dengan suhu 38,5 0C dengan tekanan CO2 5 %. Hasil penelitian menunjukkan bahwa tingkat ekspansi sel kumulus hingga tahap cumulus oocyte complexes (COCs) pada kelompok P1 tidak berbeda nyata dibandingkan kelompok P0 (p>0,05), namun kelompok P2 menunjukkan perbedaan yang nyata (p<0,05). Pada tingkat kematangan inti, penambahan hipotaurin tidak menyebabkan perbedaan yang nyata (p>0,05) pada tingkat GVBD, namun pada tingkat MI terdapat perbedaan yang nyata (p<0,05) antara kelompok P0 dengan P1 dan P2, sedangkan pada tingkat MII tidak terdapat perbedaan yang nyata (p>0,05) antara P0 dengan P1 namun berbeda nyata (p<0,05) antara P0 dengan P2. Dari hasil peneltian dapat disimpulkan bahwa penambahan hipotaurin dengan konsentrasi 4 mM pada media maturasi oosit TCM 199 dapat meningkatkan tingkat ekspansi sel kumulus mencapai COCs dan meningkatkan tingkat kematangan inti mencapai fase MII.
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