Abstract. Extreme rainfall is classically estimated using raingauge data at raingauge locations. An important related issue is to assess return levels of extreme rainfall at ungauged sites. Classical methods consist in interpolating extremevalue models. In this paper, such methods are referred to as regionalization schemes. Our goal is to evaluate three classical regionalization schemes. Each scheme consists of an extreme-value model (block maxima, peaks over threshold) taken from extreme-value theory plus a method to interpolate the parameters of the statistical model throughout the Cévennes-Vivarais region. From the interpolated parameters, the 100-yr quantile level can be estimated over this whole region. A reference regionalization scheme is made of the couple block maxima/kriging, where kriging is an optimal interpolation method. The two other schemes differ from the reference by replacing either the extreme-value model block maxima by peaks over threshold or kriging by a neural network interpolation procedure. Hyper-parameters are selected by cross-validation and the three regionalization schemes are compared by double cross-validation. Our evaluation criteria are based on the ability to interpolate the 100-yr return level both in terms of precision and spatial distribution. It turns out that the best results are obtained by the regionalization scheme combining the peaks-over-threshold method with kriging.

Dysregulation of intercellular communication is a well-established hallmark of aging. To better understand how this process contributes to the aging phenotype, we built scAgeCom, a comprehensive atlas presenting how cell-type to cell-type interactions vary with age in 23 mouse tissues. We first created an R package, scDiffCom, designed to perform differential intercellular communication analysis between two conditions of interest in any mouse or human single-cell RNA-seq dataset. The package relies on its own list of curated ligand-receptor interactions compiled from seven established studies. We applied this tool to single-cell transcriptomics data from the Tabula Muris Senis consortium and the Calico murine aging cell atlas. All the results can be accessed online, using a user-friendly, interactive web application (https://scagecom.org). The most widespread changes we observed include upregulation of immune system processes, inflammation and lipid metabolism, and downregulation of extracellular matrix organization, growth, development and angiogenesis. More specific interpretations are also provided.

Tissue fibrosis is a major driver of pathology in aging and is involved in numerous age-related diseases. The lungs are particularly susceptible to fibrotic pathology which is currently difficult to treat. The mouse bleomycin-induced fibrosis model was developed to investigate lung fibrosis and widely used over the years. However, a systematic analysis of the accumulated results has not been performed. We undertook a comprehensive data mining and subsequent manual curation, resulting in a collection of 213 genes (available at the TiRe database, www.tiredb.org), which when manipulated had a clear impact on bleomycin-induced lung fibrosis. Our meta-analysis highlights the age component in pulmonary fibrosis and strong links of related genes with longevity. The results support the validity of the bleomycin model to human pathology and suggest the importance of a multi-target therapeutic strategy for pulmonary fibrosis treatment.