Sour cherry seed flavonoid-rich extract showed a protective effect against reperfusion-induced injury through its ability to reduce the changes in concentrations of retinal ions through HO-1-related endogenous CO production in the ischemic/reperfused retina.

BackgroundThe transcription factor p63 plays a pivotal role in the development and maintenance of epithelial tissues, including the ocular surface. In an effort to gain insight into the pathogenesis of keratitis caused by HSV-1, we determined the expression patterns of the p63 and Bax proteins in the Staatens Seruminstitute Rabbit Cornea cell line (SIRC).MethodsSIRC cells were infected with HSV-1 at various multiplicities and maintained for different periods of time. Virus replication was measured by indirect immunofluorescence assay and Western blot analysis. Cell viability was determined by MTT assay. The apoptotic response of the infected cells was quantified by ELISA detecting the enrichment of nucleosomes in the cytoplasm. Western blot analysis was used to determine the levels of p63 and Bax proteins.ResultsIndirect immunofluorescence assays and Western blot analyses demonstrated the presence of HSV-1 glycoprotein D (gD) in the infected SIRC cell line, and the pattern of gD expression was consistent with efficient viral replication. The results of MTT and ELISA assays showed that HSV-1 elicited a strong cytopathic effect, and apoptosis played an important role in the demise of the infected cells. Mock-infected SIRC cells displayed the constitutive expression of ΔNp63α. The expressions of the Bax-β and TAp63γ isoforms were considerably increased, whereas the level of ΔNp63α was decreased in the HSV-1-infected SIRC cells. Experiments involving the use of acyclovir showed that viral DNA replication was necessary for the accumulation of TAp63γ.ConclusionThese data suggest that a direct, virus-mediated cytopathic effect may play an important role in the pathogenic mechanism of herpetic keratitis. By disturbing the delicate balance between the pro-survival ΔN and the pro-apoptotic TA isoforms, HSV-1 may cause profound alterations in the viability of the ocular cells and in the tissue homeostasis of the ocular surface.

VSV is a powerful inducer of apoptosis in immortalized WK cells. The VSV-mediated alterations in the expressions of Bcl-2 and Bax proteins may play important roles in the apoptotic responses of infected cells and may also sensitize to other apoptotic stimuli. This virus may possess oncolytic activity in epithelial tumors of the conjunctiva.

PURPOSE: To evaluate the results of photorefractive keratectomy (PRK) for the correction of myopia and myopic astigmatism in megalophthalmos anterior. METHODS: Four eyes of two brothers with megalophthalmos anterior were treated with PRK. In patient 1, best spectacle-corrected visual acuity (BSCVA) was 20/20 in both eyes with a refraction of -4.50 -4.50 x 180? in the right eye and -3.75 -3.00 x 175? in the left eye. In patient 2, BSCVA was 20/25 in both eyes with a refraction of -4.25 x 166? in the right eye and +0.50 -4.00 x 175? in the left eye. RESULTS: Topographic map, slit-lamp, ultrasound biomicroscopy, and postoperative course (no progression), supported with vectorial analysis, demonstrated megalophthalmos anterior. During 24-month follow-up, mild haze was observed and BSCVA was maintained. CONCLUSIONS: Myopia and astigmatism are often observed in this type of nonprogressive corneal dysgenesis. Based on this fact and our results, we recommend PRK in cases of megalophthalmos anterior. [J Refract Surg. 2006;22:408-411.]