Reaction of 1-dimethylaminonaphthalene-5-sulphonyl chloride with the secondary amines produced by denitrosation of N-nitrosamines yields dansyl-amides suitable for the mass spectrometric determination of Nnitrosamines. The method has been applied to the quantitative analysis of 3-4 component mixtures of N-nitrosamines. The detection limit was 1 ng with a relative standard deviation of 0.15-0.31. A gas chromatographic mass spectrometric method for the identification and quantification of mixtures of N-nitrosamines was also developed with a detection limit of 1 ng pl-' and relative standard deviation of 0.12-0.23. These methods are recommended for N-nitrosamine determination in environmental samples and have the advantage that the internal standard can be prepared directly from the respective secondary amine.

Children patients with alterative-exudative (gastrointestinal diseases) and allergic inflammation (bronchial asthma) exhibited similar changes in fatty acid composition of erythrocyte and leukocyte membranes. They included accumulation of considerable amounts of eicosapentaenoic acid and decrease in the content of docosahexaenoic acid (long-chain derivative of eicosapentaenoic acid). The accumulation of eicosapentaenoic acid and decrease in the content of docosahexaenoic acid in cell membranes probably play a role in the pathogenesis of chronic inflammatory diseases. It is related to a possible decrease in the formation of anti-inflammatory eicosanoids (series-5 leukotrienes) and protective compounds (resolvins and protectins) from fatty acids and changes in the physicochemical properties of cell membranes.

No abstract

No abstract