SUMMARYExperimental batches of hay were baled a t different moisture contents, and the microbial and biochemical changes studied by sequential sampling. The type of hay obtained could, in general, be related to the initial moisture content, and to the temperature subsequently attained. Good hays (about 16 yo moisture) heated little and contained a small but diverse microflora.Hays baled at about 25 oh moisture heated to about 45" and moulded, mainly with Aspergillusglaucus. Wet bales, with initial moisture contents of about $0 yo, became very hot (60°-650) and contained a large flora of thermophilic fungi, particularly Aspergillus fumigatus, Absidia spp., Mucor pusillus, Huinicola lanuginosa, and actinomycetes. During the initial heating period, which was correlated with a general rise in numbers of micro-organisms, particularly actinomycetes and bacteria, the acidity and volatile nitrogen increased. Later, when fungi and actinomycetes grew profusely, soluble sugars decreased rapidly and the pH value rose to 7-0 or above. Stacks of wet and dry hays were compared with bales made from the same hays. The wet stack developed a core of brown acid hay, containing many sporeforming bacteria but few fungi, surrounded by a layer of mouldy hay.

SUMMARY:Estimates of numbers of micro-organisms occurring in three differently manured soils, made by a direct-counting and plating technique, were compared. No correlation was found between the two methods and reasons for the large discrepancy between them are discussed. Contradictory information of the effect of external factors on soil micro-organisms can be given by the two different methods of counting.Estimates of the total numbers of bacteria in soil obtained by a direct-counting technique devised by Jones & Mollison (1948) are of the order of several thousands of millions/g. soil, while estimates by conventional plating methods are in tens of millionslg., even on the least selective media so far used. The same authors found a difference of the same kind between the quantity of fungal mycelium in soil as estimated by a direct count of number of pieces/g. as compared with colony plate counts, although in this comparison the former count is only 2-6 times greater than the latter.The effect of external factors such as soil moisture and temperature on the various groups of soil microflora has been investigated by several workers and, while certain correlations have been found, the results have been conflicting. The estimations of numbers of organisms were mainly based on plate counts. The present investigation was undertaken to compare direct counts with plate counts over a period of time, to determine how these varied with soil moisture and temperature and to show whether these two counting methods did, in their different ways, give the same sort of information about the soil population. The soils of three differently manured plots were sampled at intervals (Fig. 1) over the greater part of a year, and determinations made of the moisture content of these samples and of their bacterial, fungal and actinomycetal populations, by plating and a direct-counting technique. 'METHODSSoil sampZing. The soil samples were taken from three of the classical plots of Broadbalk field, which has grown wheat continuously for just over 100 years and whose plots have had different manurial treatments over that period.Plot 2 (farmyard manure), plot 3 (no manure) and plot 7 (complete minerals + ammonium sulphate) were sampled at intervals between January and December. Soil was collected by an auger to a depth of 4 in. in 12 areas on each plot; these were bulked and passed through a 3 mm. sieve. From these sieved samples the necessary amounts of soil were taken for the determination

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