Seven flavonol 3- O-glycosides were isolated from the flowers of CALENDULA OFFICINALIS L. Their structures were elucidated as isorhamnetin 3- O-glucoside, rutinoside, neohesperidoside, 2 (G)-rhamnosylrutinoside, quercetin glucoside, neohesperidoside, and 2 (G)-rhamnosylrutinoside by paper and thin layer chromatography, UV, (13)C-NMR, and mass spectroscopy. The interglycosidic linkages of isorhamnetin 3- O-neohesperidoside, 2 (G)-rhamnosylrutinoside, quercetin 3- O-neohesperidoside and structural determination of quercetin 2 (G)-rhamnosylrutinoside are described for the first time in CALENDULA OFFICINALIS.

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