Microdosage fluorométrique de la streptomycine et de la dihydrostreptomycine par la réaction au β-naphtoquinone-4-sulfonate de sodium en milieu alcalin

Fluorescence determination of streptomycin in serum by reversed-phase ion-pairing liquid chromatography

“…The excitation maximum of the fluorophor in the effluent from the detector was at 347 nm and the emission peak had a maximum at 445 nm. These data agreed well with those reported by Faure and Blanquet (7). Therefore, the excitation and emission detections of the fluorometer used were a 351-nm lamp and 420-nm cutoff filter, respectively.…”

“…SM containing two guanidino groups yields similar fluorescent products. The manual method has been reported for the determination of SM using NQS, but the method by HPLC using NQS has not yet been reported (7). The proposed method describes the determination of SM in serum by HPLC using NQS.…”

“…The other method does apply to the determination of SM in serum, but time-consuming sample pretreatment and a large volume (400 mL) of serum are required. This paper describes a new postcolumn fluorescence derivatization method using fl-naphthoquione-4-sulfonate, which forms fluorescent products with guanidino groups in SM (7). The method is rapid, accurate, sensitive, and specific for the determination of SM in serum.…”

Modified determination of dihydrostreptomycin in kidney, muscle and milk by HPLC†

“…1) can remain as a slowly removed antibiotic residue in animal products. Several HPLC methods based on fluorogenic post-column derivatisation of the guanidine groups of DHS with b-naphthoquinone-4-sulfonate (NQS) reagent in alkaline media [1][2][3] have been developed for the determination of DHS residues in meat 4,5 and milk. 6 This paper reports simplified and reliable sample work-up procedures and optimised HPLC and derivatisation conditions that permit detection well below the maximum residue limit (MRL) values.…”

Fluorometric Analysis

“…The native fluorescence of Antimycin A at 420 µ in water (382) and of griseofulvin in 23% ethanol (127) have been used for their respective fluorometric determinations. Although the reaction is not highly specific, streptomycin and dihydrostreptomycin can be assayed by the fluorescence resulting from treatment with 1,2naphthaquinone-4 -sulfonate (133). Spectrofluorometric analyses of blood and tissues for chlorpromazine and other phenothiazine drugs show high sensitivity, in procedures involving oxidation with permanganate (288) or 30% hydrogen peroxide (347,348).…”