The present study is to through light on role of some insecticides (chemical insecticides (a.i. chlorphyrifos) Dursban® 48% EC, spinosyns group (a.i. spinosad) Tracer® 24% SC and insect growth regulator (a.i. lufenuron) Match® 5% EC) against insect attacking cotton cultivation Spodoptera littoralis under Egyptian conditions. The toxicological effects and biochemical analysis carried out against laboratory strain of target insects after 24 and 72 hours. Toxicity of the three insecticides chlorphyrifos, spinosad and lufenuron were tested against the 2 nd instar larvae of the cotton leafworm, spodoptera littoralis. The results showed that lufenuron was more effective on the 2 nd instar larvae than chlorphyrifos and spinosad. The LC25 values for lufenuron, chlorphyrifos and spinosad were 0.0005, 2.21 and 8.1 ppm, respectively. The biochemical study used for tested insecticides at LC25 on some biocomponents namely acetylcholinesterase (AchE), glutathione-s-transferase (GST), chitinase, phenoloxidase, aspartate (AST) and alanine (ALT) aminotransferases, α-esterase (α-EST), total protein and protein gel electrophoresis for laboratory strain were investigated. The tested chlorphyrifos and lufenuron were significantly increased (AchE). Spinosad was significantly decreased (GST) and phenoloxidase. Spinosad and lufenuron significantly increased (α-EST) and (chitinase) (ALT). Chlorphyrifos caused significantly decreased on α-esterase and total protein and (AST). Lufenuron significantly decreased total protein and phenoloxidase. Spinosad showed insignificant increase in (AchE) and total protein (levels). Chlorphyrifos decreased both of phenoloxidase and (ALT) levels insignificantly and increased the levels of (GST) and chitinase, while lufenuron, recorded insignificant decrease in GST levels. The total body proteins of 2 nd instar larval of S. littoralis treated with LC25 of insecticides performed by SDS-PAGE. Control and different treatments were separated into 56 different bands according to their relative frequencies (Rf values), and molecular weights (MW). Samples of electrophoresis were carried out for three different insecticides namely chlorphyrifos, spinosad and lufenuron which used to treat insects. The treatment with insecticides on protein gel electrophoresis led to detection of new bands, and disappeared some bands in comparison to control. It is concluded that treatment with insecticides have strong efficacy on the soluble protein in the body of insects.

In this study, alcoholic and aqueous extracts of rosemary and its oil were used to investigate their effects on diabetic and liver malfunctional rats by administrating them with STZ or CCl4 for three weeks. The therapeutic effects of rosemary extracts (alcoholic and aqueous) and its oil against acute hyperglycemia induced by STZ in rats showed a decrease in the levels of fasting and postprandial blood glucose of rats treated with alcoholic extract of rosemary. In addition, results showed a restoration of these levels of the rats treated with aqueous extract or oil to their normal ranges after 3 weeks. There is a significant difference in lipid profile between rats treated with alcoholic or aqueous extracts and the negative control, where, the HDL in group administrated rosemary oil is significantly higher than both positive and negative control. The protective effects of rosemary extracts (alcoholic and aqueous) and its oil against liver toxicity induced by CCl4 in rats showed a significant difference between both (negative and positive control) and the activity of AST and ALT in the rats treated by alcoholic and aqueous extracts of rosemary. The protective effects of the plant extracts against liver toxicant occurred by decreasing the activities of these enzymes. Oil rosemary had restored effect more than both ethanolic and aqueous extracts, but not significant. This protective effect occurred by decreasing the activities of AST, ALT and Alp enzymes. No significant (p≤0.05) differences were noticed among the levels of serum total protein for all treatments compared to the negative and positive controls. The levels of total cholesterol and triglycerides recorded high values for the positive controls compared to the negative control. The protective effects of rosemary extracts against liver toxicant occurred by restoring the levels of these parameters to their normal values.

Hyperprolactinaemia is the occurrence of an endocrine disorder that leads to an increase in the level of the hormone prolactin (PRL) in the blood above normal levels in cases other than the natural increase in pregnancy and lactation. It may be present as the menstrual disturbances, galactorrhea, sexual dysfunction, gynecomastia, infertility, decreased bone mineral density, and breast cancer. This increase results from several causes that may be pathological such as pituitary tumors, psychological such as stress, or as a result of taking a specific drug such as schizophrenia, anti-vomiting drugs, nerve analgesics, first-generation antipsychotics, hypnotics, and sedatives. Hyperprolactinaemia by metoclopramide was induced in male and female rats to assess the therapeutic effects of alcoholic extracts of sage leaves, marjoram leaves and celery seeds. Through the biochemical analysis of blood hormones, the results showed that treating rats with metoclopramide drug (150 mg / kg / day) for a week had an effect on the positive control group in the occurrence of hyperprolactinaemia compared to the negative control group at the beginning of the experiment. The results indicated that there was a significant effect (P<0.05) for the three plant extracts namely marjoram leaves, sage leaves and celery seeds ( 250 mg / kg / day) for 30 days, to reduce the prolactin hormone in the blood and also to maintain the level of the rest of the sex hormones (progesterone (PRG), estrogen (E2), testosterone (TST), Follicle-stimulating hormone (FSH) and Luteinizing hormone (LH)) without causing any disturbance in males and females in comparison to the group of drug used in the experiment. To treat this condition bromocriptine (Dopamine receptors agonist) is used. The rats returned to normal case after the appearance of the rats' recovery after taking the extracts during the trial period. This effect may be due to estrogen receptors and /or dopamine receptor.

Hyperuricemia (elevated serum levels of uric acid) is a key risk factor for the development of gout, and has been linked to renal dysfunction, cardiovascular diseases, hypertension, diabetes and metabolic syndrome. Hyperuricemia was induced by oxonic acid (uricase inhibitor

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