Plant uptake is one of the major pathways by which toxic metals enter to human food chain. The irrigation water, important source of toxic metals, is contaminated with effluent and other pollutants due to the widespread industrialization. Two lyzimeter experiments were conducted at Sakha Agric. Res. Station, Kafr El-sheikh, Egypt, for two successive seasons, 2010 and 2011.It aimed to study the effect of irrigation water quality for long-term on productivity of four cotton genotypes; Giza86, Giza87, Giza88 and Giza89 in the two seasons were to study the content of their root, stem, leaves, and seed cotton of heavy metals; Cd, Ni and Pb as affected by irrigation water quality. Lyzimeters (100\ 70/ 90 cm) were filled with clayey soil was irrigated with three water qualities since 1987. They were W1, Nile water, W3, drainage water and W2, mixed water, 50% W1+50%W3. A split-plot design with four replicates were used where, irrigation water quality and cotton genotype were allocated to main and subplots, respectively .The obtained results showed that using drainage water quality for irrigation increased ECe, SAR, soluble Na + , Mg ++ , So4 = and Cl in soil paste extract, total and available Pb,Cd and Ni than that of mixed or Nile water. The results show also that the effect of genotypes were different significantly for all studied cotton yield and same characters of tested genotypes .The effect of drainage water on studied genotypes were significantly different on most studied characters; the highest values for seed cotton yield , seed and plant high were obtained from Nile water followed by mixed water mean while drainage water gave the lowest values for this characters .The interaction between water qualities and cotton genotypes were significant in some studied characters. In general, stem cotton of the studied heavy metals were higher than that of root, leaves, and seed cotton for all water quality treatments .Cotton root, stem, leaves, and seed cotton contents of Pb ,Cd and Ni greater when drainage water (W3) was applied than that of the mixed or Nile water.

Canola Brassica napus Seru-4 variety was grown in lyzimeter experiment at Sakha Agric. Res. Station during 2008/2009 season to study the metal accumulation and it's feasibility of it's use for metal phytoextraction. Also, chosen to study the content of roots, stems, leaves, seeds and oils of heavy metals Mn, Cu, Zn, Ni, Cd and Pb as affected by water treatments Lyzimeters (100 x 70 x 90 cm) were field with clayey soil and irrigated with three water treatments since 1987 twenty years ago. They were W1, Nile water (good water quality) and W3, drainage water (relatively poor water quality) and W2, mixed water 50% W1 + 50% W3. Complete randomized block design with four replicates was used for statistical analysis. The obtained results showed that:  Using poor water quality for irrigation increased ECe, SAR, soluble cations and anions in soil paste extract and total DTPA extractable heavy metals in soils (Mn, Zn, Cu, Ni, Cd and Pb) than that of mixed or good water quality.  No significant effect of water treatment was found on plumule length swelling coefficient, hydration coefficient, crude fat, ash, relative density percent of canola seeds.  The best main radical length and crude protein were obtained in seeds of canola plants irrigated with drainage water.  Content of the studied heavy metals were in the following order: roots > stems > leaves > seeds > oils and greater when poor water quality (W3) was used for irrigation water.  Highly significant differences were found between means of heavy metal content in canola plant due to irrigation water treatment.  Canola oils which extracted from seeds were the least content of studied heavy metals in canola plant organs.  Canola behaved as an accumulator for heavy metals such as Zn, Mn, Cd, Ni and Pb and is useful for metal phytoextraction due to higher metal accumulation capacity.

Two field experiments were conducted during two successive seasons 2006 and 2007 at the experimental farm of Sakha Agric. Rec. Station, Kafer El-Sheikh Government. The aim of this study was to investigate the influence of zinc addition 0, 10, and 20 Kg/ fad. For Zn0(S) (control), Zn10(S), and Zn20(S), respectively or foliar application with or without urea, 2% urea [U(F)], 500 ppm Zn [Zn (F)] and 2% urea + 500 ppm Zn [U+ Zn ( F)], respectively under different levels of phosphorus fertilization 30, 45, and 60 Kg P2O5 for P1, P2, and P3 respectively on maize yield and its chemical composition. Ratherfore soil content of P , Zn , Fe, and Mn. The experiments were conducted in split plot design where P levels were the main plot and Zn treatments as were sub plot with four replicates. The obtained results can be summarized as follows: The yield and its components of maize were significantly affected by P and Zn fertilizer treatments.  Application of P3 increased grain yield by 12.0 and 12.5% and biomass by 17.6 and 13.8 compared to control treatment (P1) in 2006 and 2007 seasons.  Soil application of 10 Kg zn / fad. under P3 level gave the highest value of grain yield in the two seasons. and of the biomass in the first season, meanwhile [U+ Zn(F)] treatment gave the highest biomass value in the second season. While, the highest value of 1000 grain weight were obtained by [Zn0 (S)] and [U+ Zn (F)] treatments in the two seasons.  The maximum values of P maize grain content were obtained by Zn20(S) treatment under P3 and P2 in the two seasons respectively.  the maximum values of P maize stem content were obtained by [Zn10(S )]and [Zn (F)] treatments under P2 in the two seasons , respectively.  The maximum values of zn maize grain content were obtained by [U+ Zn (F)] treatment under P2 level, while [Zn (F)] treatment gave the maximum maize stem zn content under P3 level in the two seasons.  The maximum values of maize grain and stem Fe content were obtained by application of [U+ Zn(F)] treatment under P1 level in the two seasons except grain in the first season. The same treatment also gave the maximum values of Mn of maize grain and stem under P3 level except the stem in the first season.  Translocation coefficient (TC%) of heavy metal from stem to grain can be arranged in the following decreasing sequence Zn > Mn> Fe.  Available P, Fe, and Mn increased by increasing P fertilizer levels from P1 to P3, while available Zn increased by increasing P fertilizer levels from P1 to P2 but at P3 it decreased.  [U+ Zn (F)] treatment gave the highest available P, Fe, and Mn, while the highest available Zn was obtained by [Zn20(S)] treatment.

A pot experiment was conducted during winter season of (2013/2014) at Sakha Agricultural Research Station, Kafr El-Sheikh Governorate. The experiment aimed to investigate the effect of disodium ethylenediaminetetraacetic acid (EDTA) (0.0, 1.5, 3.0 and 4.5 mmol kg-1 soil), citric acid (CA) (0, 3, 6 and 9 mmol kg-1 soil) and humic acid (HA) (0.0, 0.2, 0.4 and 0.6 g kg-1 soil) on the phytoextraction of Cu, Zn and Pb from Al-Gabal Al-Asfar contaminated soil using indian mustard (Brassica juncea) and the leaching behavior of these metals from soil. The obtained results can be summarised in the following:-EDTA severely reduced indian mustard dry weight and visual symptoms of toxicity were observed, especially at 4.5 mmol EDTA kg-1 soil. Whilst, application of CA and HA ameliorated indian mustard growth.-EDTA was found to be the most efficient chelator in increasing concentrations of Cu and Zn in different indian mustard plant parts.-Increasing EDTA application rate up to 4.5 mmol kg-1 soil increased the concentrations of Cu by 1.91, 2.88, 3.04 and 1.56 folds and Zn by 1.43, 1.91, 1.83and 1.11 folds for roots, stem, leaves and seeds, respectively, compared to control treatment (0.0 mmol EDTA kg-1 soil).-Application of CA also increased Cu and Zn concentrations by different indian mustard plant parts, especially at 9 mmol kg-1 soil, but its efficacy was less than EDTA.-HA application increased Zn concentrations in different indian mustard plant parts with increasing its addition up to 0.6 g kg-1 soil, but its efficacy was less than that of EDTA and CA.-HA application did not affect Cu concentrations in indian mustard.-There were neither any perceptible concentrations of Pb in different plant parts of indian mustard except for roots clarifying that phytoextraction of Pb failed even after application of tested chelators.-Application of EDTA and CA to the soil increased the leaching of Cu, Zn and Pb under indian mustard cultivation with increasing application rate up to 4.5 and 9 mmol kg-1 soil, respectively. However, the effect of EDTA was many times higher than that of CA. Whilst, HA application did not affect the leaching of studied metals.-Using CA for the phytoremediation of Cu and CA and HA for the phytoremediation of Zn is favorable than EDTA despite the high efficiency of EDTA, due to the harmful effects of EDTA on plant growth and the increased risk of groundwater contamination via metal leaching.