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TLC identification of presumptive ochratoxins A and B can be supplemented with overnight results obtained by a single and rapid bioassay of these toxins, using Bacillus cereus mycoides LSU as the test organism. Furthermore, this bioassay can provide an accurate quantitative estimation of ochratoxins. The calculated coefficient of variation was 5.9%. The optimal medium, conditions, and ochratoxin concentrations for this assay are suggested. This test is sensitive to as little as 1.5 and 3.0 µg ochratoxins A and B, respectively.