This study examined the phytotoxic impact of transmission oil pollution on Oat (Aveena sativa) seedlings growing in contaminated soils under 3 different soil moisture conditions of 100% water saturation, 60% and 30% water contents. The synergistic role of the microorganism Pseudomonas putida as a bioremediating agent was also examined.Experimental soil was treated with transmission oil for up to 12 weeks but seedlings were grown and measured on weeks 1, 7 and 12 using derno-podzoluivisolic soil collected from forest reserve of the Russian State Agrarian University. Results showed that seedlings grown on week 7 and 12 germinated better but had variable growth rates. In addition, Pseudomonas putida seemed to improve seedling germination at lower water contents for both weeks. This experiment suggests that Pseudomonas putida may be a good candidate for bioremediation.